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BRAIN CONNECTS: High-Throughput Single Cell Projection Mapping and Spine Quantification in the Primate Brain by Synaptic Barcoding

Award Number: U01NS136398
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/31/2024
PERIOD OF PERFORMANCE END DATE: 07/31/2027

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BRAIN CONNECTS: High-Throughput Single Cell Projection Mapping and Spine Quantification in the Primate Brain by Synaptic Barcoding - SUMMARY The primate brain contains trillions of highly specific synaptic connections that are the building blocks of neural computation. While tools available in rodents have begun to shed light on how particular cell types and circuits participate in brain functions, these approaches are not currently possible to apply to primates. Recently, our labs have developed technology that: 1) delivers barcoded RNAs to the presynaptic and postsynaptic compartments with high sensitivity and specificity using AAVs; and 2) allows CNS-wide transduction by AAV through systemic injection. We will combine these innovations, together with our platforms for single-cell and spatial transcriptomics, to systematically map the projections of thousands of midbrain dopaminergic neurons across the marmoset brain. In addition, we will develop strategies for the rapid and scalable quantification of postsynaptic spines in individual cells, using known abundance in spine variation amongst the cells of the dentate gyrus as a validation. Together, these technologies will enable the routine quantification of presynaptic and postsynaptic phenotypes at a level of resolution and scale that has hitherto been impossible in primate


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $1,853,811 )
2025	2025	THE BROAD INSTITUTE, INC	415 MAIN ST	CAMBRIDGE	MA	02142	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	6/25/2025	NON-COMPETING CONTINUATION	$1,853,811
														Subtotal = $1,853,811

Issue Date FY: 2024 ( Subtotal = $1,481,203 )
2024	2024	THE BROAD INSTITUTE, INC	415 MAIN ST	CAMBRIDGE	MA	02142	MIDDLESEX	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/30/2024	NEW	$1,481,203
														Subtotal = $1,481,203

Grand Total All Awards = $3,335,014

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BRAIN CONNECTS: High-Throughput Single Cell Projection Mapping and Spine Quantification in the Primate Brain by Synaptic Barcoding

Award Number: U01NS136398

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/31/2024

PERIOD OF PERFORMANCE END DATE: 07/31/2027

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