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Novel Methodologies for the Preservation (and Recovery) of Proteins at Low Temperature

Award Number: SC3GM139673
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/02/2021
PERIOD OF PERFORMANCE END DATE: 08/02/2024

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Novel Methodologies for the Preservation (and Recovery) of Proteins at Low Temperature - ABSTRACT The goal of this SC3 grant is to develop a novel method for the cryopreservation of proteins without the use of co-solvents, which are usually employed to limit the structural changes that can occur during freezing. The method exploits the glass polymorphism of water, i.e., low density amorphous (LDA) and high density amorphous (HDA) glasses can be produced following specific cooling/compression pathways. The pathways for storage and recovery of proteins will be studied using non-equilibrium molecular dynamic simulations coupled to all-atom force fields. The central hypothesis is that the density of the HDA will limit fluctuations in the solution upon cooling and the protein will not undergo any significant structural changes when vitrified in HDA. The rationale for the proposed research is that an understanding of the detailed mechanism by which proteins are preserved in glassy water, can lead to the rational design of experiments that allow for more controlled subzero protein preservation. In order to systematically develop this understanding three groups of systems, with increasing order of structural complexity will be studied: (i) short peptides with different secondary structures (ii) simple proteins that contain these secondary structures within their tertiary structures, and (iii) two biomedically relevant proteins. The information obtained from these studies has the potential to be extended to the storage of pharmaceuticals, food, and biological cells without the use of co-solvents.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = -$253,637 )
2025	2024	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD W	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	000	4	10/16/2024	NON-COMPETING CONTINUATION	$0
2025	2024	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD W	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	003	4	1/7/2025	NON-COMPETING CONTINUATION	-$119,120
2025	2023	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD W	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	002	3	1/7/2025	NON-COMPETING CONTINUATION	-$119,120
2025	2022	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD W	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	001	2	1/7/2025	NON-COMPETING CONTINUATION	-$15,397
														Subtotal = -$253,637

Issue Date FY: 2024 ( Subtotal = $119,120 )
2024	2024	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD W	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	000	4	9/16/2024	NON-COMPETING CONTINUATION	$119,120
														Subtotal = $119,120

Issue Date FY: 2023 ( Subtotal = $119,120 )
2023	2023	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	000	3	7/25/2023	NON-COMPETING CONTINUATION	$119,120
														Subtotal = $119,120

Issue Date FY: 2022 ( Subtotal = $119,120 )
2022	2022	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	000	2	8/5/2022	NON-COMPETING CONTINUATION	$119,120
														Subtotal = $119,120

Issue Date FY: 2021 ( Subtotal = $119,120 )
2021	2021	RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK	250 BEDFORD PARK BLVD	BRONX	NY	10468	BRONX	USA	Biomedical Research and Research Training	000	1	8/2/2021	NEW	$119,120
														Subtotal = $119,120

Grand Total All Awards = $222,843

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Novel Methodologies for the Preservation (and Recovery) of Proteins at Low Temperature

Award Number: SC3GM139673

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/02/2021

PERIOD OF PERFORMANCE END DATE: 08/02/2024

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