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Monitoring the three-dimensional motion of chromatin at single molecule resolution

Award Number: R35GM147412
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/20/2022
PERIOD OF PERFORMANCE END DATE: 06/30/2027

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Monitoring the three-dimensional motion of chromatin at single molecule resolution - PROJECT SUMMARY The notion that the positions of chromosomal loci within the interphase nucleus are not static, but move, is not new. However, the mechanistic role of the chromatin motion is usually underestimated. In fact, the nanoscale motion of chromatin may modulate the interaction of DNA with regulatory molecules, including chromatin effectors, transcription factors and non-coding RNAs, thus impacting the global patterns of gene expression. Unfortunately, experimental evidence supporting an essential role of chromatin motion in these activities is sparse. Thus, it is unclear if changes in chromatin dynamics facilitate these biological processes or are simply consequences. The goal of this project is to quantify the chromatin motion throughout the mammalian nucleus and to explore its mechanistic role in genome functions. To this end, the proposed research combines multidisciplinary approaches to concentrate on the link of chromatin motion with epigenetic regulation, DNA damage, and transcription, with the ultimate goal to understand the causality from epigenetic modification to phenotypic gene expression. The proposed projects are listed as below, Project 1 Map the chromatin motion in 3D with nanometer resolution. We will heavily incorporate the data science approach into our innovative imaging system to optimize the toolbox of measuring the chromatin. The toolbox will include the data-driven 3D imaging system and data science supported image informatics software. Project 2 Investigate the link between chromatin motion and epigenetic modification and transcription. We will explore the reciprocal interactions between chromatin motion and epigenetic modifications as well as transcription, respectively. These experimental investigations can feed proper mathematical models, therefore to formalize the reaction network in chromatin remodeling and gene expression. Results generated from these projects have great potential to reveal fundamental links between chromatin motion and gene expression, which will transform our understanding of disease mechanism and facilitate the development of therapeutic intervention.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $382,987 )
2025	2025	PURDUE UNIVERSITY	2550 NORTHWESTERN AVE # 1100	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Biomedical Research and Research Training	000	5	6/20/2025	NON-COMPETING CONTINUATION	$382,987
														Subtotal = $382,987

Issue Date FY: 2024 ( Subtotal = $335,570 )
2024	2024	PURDUE UNIVERSITY	2550 NORTHWESTERN AVE # 1100	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Biomedical Research and Research Training	005	4	7/25/2024	NON-COMPETING CONTINUATION	$386,127
2024	2023	TRUSTEES OF INDIANA UNIVERSITY	980 INDIANA AVE RM 2232	INDIANAPOLIS	IN	46202	MARION	USA	Biomedical Research and Research Training	000	2	7/11/2024	NON-COMPETING CONTINUATION	-$322,838
2024	2023	TRUSTEES OF INDIANA UNIVERSITY	980 INDIANA AVE RM 2232	INDIANAPOLIS	IN	46202	MARION	USA	Biomedical Research and Research Training	003	2	7/22/2024	NON-COMPETING CONTINUATION	-$66,657
2024	2023	PURDUE UNIVERSITY	2550 NORTHWESTERN AVE # 1100	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Biomedical Research and Research Training	004	3	7/22/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$100,397
2024	2023	PURDUE UNIVERSITY	2550 NORTHWESTERN AVE # 1100	WEST LAFAYETTE	IN	47906	TIPPECANOE	USA	Biomedical Research and Research Training	001	3	7/12/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$272,281
2024	2022	TRUSTEES OF INDIANA UNIVERSITY	980 INDIANA AVE RM 2232	INDIANAPOLIS	IN	46202	MARION	USA	Biomedical Research and Research Training	002	1	7/22/2024	NEW	-$33,740
														Subtotal = $335,570

Issue Date FY: 2023 ( Subtotal = $389,495 )
2023	2023	TRUSTEES OF INDIANA UNIVERSITY	980 INDIANA AVE RM 2232	INDIANAPOLIS	IN	46202	MARION	USA	Biomedical Research and Research Training	000	2	6/27/2023	NON-COMPETING CONTINUATION	$389,495
														Subtotal = $389,495

Issue Date FY: 2022 ( Subtotal = $351,715 )
2022	2022	TRUSTEES OF INDIANA UNIVERSITY	980 INDIANA AVE RM 2232	INDIANAPOLIS	IN	46202	MARION	USA	Biomedical Research and Research Training	000	1	9/19/2022	NEW	$351,715
														Subtotal = $351,715

Grand Total All Awards = $1,459,767

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Monitoring the three-dimensional motion of chromatin at single molecule resolution

Award Number: R35GM147412

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/20/2022

PERIOD OF PERFORMANCE END DATE: 06/30/2027

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