Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Single-Particle Analysis of Virus Capsids, Bacteria, and Extracellular Vesicles

Award Number: R35GM141922
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/01/2021
PERIOD OF PERFORMANCE END DATE: 02/28/2031

Group Awards By:

View Award Description

Single-Particle Analysis of Virus Capsids, Bacteria, and Extracellular Vesicles - Project Summary We are developing micro- and nanofluidic devices to probe virus capsids, bacteria, and extracellular vesicles at the single-particle level with improved spatial and temporal resolution. We are targeting single-particle measurements, which can significantly impact the overall function or fate of a system, because these events are often obscured when measurements are made on bulk samples. Information content is further enhanced by performing these assays in a high-throughput, multiplexed format, where individual events are tracked, but population statistics are also obtained. In the first project, we are studying virus capsid assembly and disassembly. To monitor reactions with capsids, we are designing in-plane nanofluidic devices with multiple nanopores in series for resistive-pulse sensing, which is a label-free, nondestructive sizing technique. Resistive-pulse sensing detects events in real time and has sufficient sensitivity to monitor assembly at biologically relevant concentrations and over a range of reaction conditions. With these nanofluidic devices, we are evaluating how capsid assembly modulators and chaotropes impact the assembly process and produce a variety of particle morphologies, including kinetically trapped intermediates and aberrant structures. In the second project, we are developing complementary methods for biophysical characterization of EVs derived from various cell lines. To determine the size, shape, and surface-charge density of EVs, we use resistive-pulse sensing, and to test the rigidity of the lipid bilayer, we couple the resistive-pulse sensing (RPS) with fluorescence lifetime imaging microscopy (FLIM). The in-plane format of our devices makes coupling the RPS with FLIM straightforward. In the third project, we are tracking development and aging of bacteria with microfluidic devices that have integrated microchannel arrays to physically trap bacteria. The microchannels confine growth of bacteria in one dimension, and when coupled with fluorescence microscopy, image analysis is reduced from a three-dimensional to one-dimensional problem and greatly simplified. Growth and division rates, subcellular functions, and epigenetic effects are easily tracked for extended periods of time and across multiple generations.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $669,537 )
2026	2026	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	6	6/18/2026	COMPETING CONTINUATION	$669,537
														Subtotal = $669,537

Issue Date FY: 2025 ( Subtotal = $539,920 )
2025	2025	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	001	5	7/15/2025	NON-COMPETING CONTINUATION	$539,920
2025	2024	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	4	11/18/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $539,920

Issue Date FY: 2024 ( Subtotal = $542,047 )
2024	2024	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	4	5/20/2024	NON-COMPETING CONTINUATION	$542,047
														Subtotal = $542,047

Issue Date FY: 2023 ( Subtotal = $544,071 )
2023	2023	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	3	6/7/2023	NON-COMPETING CONTINUATION	$544,071
														Subtotal = $544,071

Issue Date FY: 2022 ( Subtotal = $545,998 )
2022	2022	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	2	5/31/2022	NON-COMPETING CONTINUATION	$545,998
														Subtotal = $545,998

Issue Date FY: 2021 ( Subtotal = $626,355 )
2021	2021	TRUSTEES OF INDIANA UNIVERSITY	107 S INDIANA AVENUE	BLOOMINGTON	IN	47405	MONROE	USA	Biomedical Research and Research Training	000	1	5/26/2021	NEW	$626,355
														Subtotal = $626,355

Grand Total All Awards = $3,467,928

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Single-Particle Analysis of Virus Capsids, Bacteria, and Extracellular Vesicles

Award Number: R35GM141922

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/01/2021

PERIOD OF PERFORMANCE END DATE: 02/28/2031

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer