Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Unsupervised optimization of protein therapeutics using closed-loop in vitro synthesis, nanosensing,and deep-learning

Award Number: R35GM138265
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/05/2020
PERIOD OF PERFORMANCE END DATE: 12/31/2030

Group Awards By:

View Award Description

Unsupervised optimization of protein therapeutics using closed-loop in vitro synthesis, nanosensing,and deep-learning - PROJECT SUMMARY / ABSTRACT Overview of research in the laboratory – The Reuel Group is focused on sensor and probe development to provide data necessary for machine learning (ML) enabled optimization of biologic products and processes. Active learning techniques, such as reinforcement learning, can discover new materials and methods but are reliant on large, standardized data sets that can be efficiently and iteratively collected in the lab. For the optimi- zation of protein products (MIRA project focus) fluorescent nanoprobes built off single walled carbon nanotubes (SWCNT) are used to rapidly characterize proteins prototyped in cell free expression. For the optimization and control of enzyme production in fermentation (BioMADE project) SWCNT probes are used on sampled fermen- tation broth. For the optimization of cell therapy manufacturing (NSF CAREER and EPSCoR projects) radio frequency based resonant sensors and imaging are used to track cell expansion and differentiation. To make progress in these fields, the group has built up capabilities to test and establish whole workflow solutions, includ- ing the capacity to make and design new sensors, conduct high throughput cell and cell free biologic experiments with robotic fluid handlers, and apply the latest active learning algorithms to these data to generate new protein sequences and control cell production process parameters. Goals for the next five years – The Reuel Group aspires to produce materials and methods for ML assisted biologic product discovery and manufacturing that are simple, robust, and straightforward to use, such that any lab wishing to make a new custom protein or cell production process would be able to use these powerful tech- niques. The methods and workflows will be described in publications, but most of the resources will also be embedded into accessible web interfaces where one would specify a target, be directed to the necessary exper- iment to run, upload data for analysis, and then receive generated next experiments until convergence on a new optimized product is achieved. To support the experiments charted through this web interface, the reagents from this work will be made commercially available through licensing or new company formation. Overall vision of the research program – Biology is the solution to many of the world’s pressing health and sustainability needs. We firmly believe that new cell and protein-based products can be used to cure diseases, improve nutrition, track health, and provide sustainable fuels, food, and materials. Natural and directed evolution has provided a wealth of cell and protein products that are used currently with great impact. Now, with the advent of high-performance computing and AI techniques, new design spaces, yet explored by Nature, can be effectively searched. The Reuel Group envisions the need for large, iterative data sets to support this exploration and is working to that end through this MIRA support to design the foundational materials and protocols necessary. These will then be leveraged into follow-on, collaborative research projects by our group and made available to the world to support new protein design.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $376,474 )
2026	2026	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	001	6	3/12/2026	COMPETING CONTINUATION	$376,474
2026	2024	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	5	3/6/2026	TERMINATION	$0
														Subtotal = $376,474

Issue Date FY: 2024 ( Subtotal = $361,517 )
2024	2024	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	5	9/11/2024	NON-COMPETING CONTINUATION	$361,517
														Subtotal = $361,517

Issue Date FY: 2023 ( Subtotal = $525,052 )
2023	2023	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	001	4	8/28/2023	SUPPLEMENT FOR EXPANSION	$162,689
2023	2023	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	515 MORRILL RD, 1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	4	7/26/2023	NON-COMPETING CONTINUATION	$362,363
														Subtotal = $525,052

Issue Date FY: 2022 ( Subtotal = $363,174 )
2022	2022	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	515 MORRILL RD, 1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	3	9/6/2022	NON-COMPETING CONTINUATION	$363,174
														Subtotal = $363,174

Issue Date FY: 2021 ( Subtotal = $363,953 )
2021	2021	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	515 MORRILL RD, 1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	2	7/12/2021	NON-COMPETING CONTINUATION	$363,953
														Subtotal = $363,953

Issue Date FY: 2020 ( Subtotal = $327,666 )
2020	2020	IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY	515 MORRILL RD, 1350 BEARDSHEAR HALL	AMES	IA	50011	STORY	USA	Biomedical Research and Research Training	000	1	9/2/2020	NEW	$327,666
														Subtotal = $327,666

Grand Total All Awards = $2,317,836

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Unsupervised optimization of protein therapeutics using closed-loop in vitro synthesis, nanosensing,and deep-learning

Award Number: R35GM138265

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/05/2020

PERIOD OF PERFORMANCE END DATE: 12/31/2030

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer