Thursday, April 3, 2025 4/3/2025

The genetic and epigenetic mechanisms of phenotypic innovationhttps://apps.era.nih.gov/gm/reportCheckList.do?applicationID=9798249

Award Number: R35GM133780
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Group Awards By:

View Award Description

The genetic and epigenetic mechanisms of phenotypic innovationhttps://apps.era.nih.gov/gm/reportCheckList.do?applicationID=9798249 - PROJECT SUMMARY The goal of this research proposal is to dissect the novel regulatory circuits and genes underlying novel traits, to get a better understanding of the genetic basis of morphological and cellular innovation. Every morphological structure or trait originated at some time point in the past and evolved under various evolutionary paths. However, it is unknown how a novel trait originates and how gene and regulatory networks spatially orchestrate the development of the novel cell types, tissues, and organs. Identifying the processes driving and governing morphological and functional diversity and complexity is a major step towards understanding the evolution of complex life. However, our understanding of this process is still limited. The long-term goal of this research program is to functionally characterize the molecular genetic basis of novel cell clusters and novel morphological phenotypes. The central hypothesis is that evolutionary innovations emerging from novel regulatory networks depend on changes in transcription factors and enhancers. Guided by preliminary data including single cell RNA sequencing and well-established theories, the proposed research will test the central hypothesis using an integrative approach. We will determine: 1) regulatory network innovation in novel cell clusters in Drosophila, 2) enhancers responsible for transcription factor expression changes and downstream expression network modification, and 3) the genetic regulatory basis of a novel trait. We performed single-cell sequencing and RNA fluorescent in situ hybridization (FISH) on testis and found a novel cell cluster differentiated between Drosophila species. Combined with ATAC-sequencing data, we will use modeling and functional studies to study the genetic basis of the novel cell cluster. Following this hypothesis that novel enhancers of transcription factors (TFs) are essential for novel traits, we will study the cause of a recurrent novel trait and test the hypothesis that a novel regulatory circuit is essential for a novel trait. We hypothesize that novel enhancers or cis-regulatory motifs of TFs are essential for whole-genome level changes in chromatin accessibility. To test it, we will identify enhancer and motif changes between closely related species to provide insights into enhancer and TF binding affinity co- evolution. This study will provide important insights into the evolution of transcription regulatory networks and their contributions to novel morphological and cellular traits. Altogether, our integrative approach will help to elucidate the origination and evolution of novel regulatory circuits and their contributions to phenotypic innovation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	6	11/5/2024	COMPETING CONTINUATION	$0
2025	2023	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	001	5	3/4/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $457,650 )
2024	2024	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	6	8/2/2024	COMPETING CONTINUATION	$457,650
														Subtotal = $457,650

Issue Date FY: 2023 ( Subtotal = $423,703 )
2023	2023	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	5	6/7/2023	NON-COMPETING CONTINUATION	$423,703
														Subtotal = $423,703

Issue Date FY: 2022 ( Subtotal = $423,703 )
2022	2022	ROCKEFELLER UNIVERSITY	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	4	5/19/2022	NON-COMPETING CONTINUATION	$423,703
														Subtotal = $423,703

Issue Date FY: 2021 ( Subtotal = $423,703 )
2021	2021	ROCKEFELLER UNIVERSITY, THE	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	3	6/15/2021	NON-COMPETING CONTINUATION	$423,703
														Subtotal = $423,703

Issue Date FY: 2020 ( Subtotal = $423,703 )
2020	2020	ROCKEFELLER UNIVERSITY, THE	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	2	6/5/2020	NON-COMPETING CONTINUATION	$423,703
														Subtotal = $423,703

Issue Date FY: 2019 ( Subtotal = $423,703 )
2019	2019	ROCKEFELLER UNIVERSITY, THE	1230 YORK AVE	NEW YORK	NY	10065	NEW YORK	USA	Biomedical Research and Research Training	000	1	8/26/2019	NEW	$423,703
														Subtotal = $423,703

Grand Total All Awards = $2,576,165

Top

All Categories

About

Search

Reports

Data Submission

Award Information

The genetic and epigenetic mechanisms of phenotypic innovationhttps://apps.era.nih.gov/gm/reportCheckList.do?applicationID=9798249

Award Number: R35GM133780

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer