Thursday, November 21, 2024
11/21/2024
We request an NIGMS Administrative Supplement for Equipment (NOT-GM-24-021/PA-20- 272) for R35GM127059, our MIRA entitled “Morphogenesis: Biophysics and Genetics of Dorsal Closure”. This equipment supplement will provide funds to replace selected, outdated components of the 22 year old Zeiss AxioImager.M2m UV surgical confocaling microscope system that is the mainstay of our biophysical investigation of embryo development and contributes to time-lapsed analysis of dorsal closure mutations discovered through forward genetic screens. The new components that we seek to acquire using funds from our parent MIRA, from Duke Institutional resources and from this requested supplement are as follows. · A state of the art, confocaling head that can capture a wider field of view and with higher resolution than our current system, which, because of old technology does not adequately record images at full resolution (to be funded by this requested Supplement). · An ORCA-Quest 2 low noise camera that has a large (4096 x 2304 pixel) qCMOS imaging sensor, allows high frequency imaging and has increased resolution due to small pixel size (to be funded by our parent MIRA). · Two new, high NA objectives for biophysical surgical experiments and time-lapsed analysis of development that are optimized for thicker biological specimen (e.g. embryos, to be funded by Duke Institutional resources). · A TriggerScope digital control box that interfaces the lasers and the acquisition software to allow for high-speed triggering of the lasers and synchronization of the lasers with the camera exposures (to be funded by our Parent MIRA). · A Windows 11 based computer to control the system hardware, run the image acquisition software, handle large file sizes and enable compliance with institutional security requirements (to be funded by our Parent MIRA). These components will modernize and expand our current imaging capabilities that are currently, “throwing data” away. The upgraded system will provide increased resolution and field of view and in combination with our existing computer steered laser UV microbeam for laser microsurgery, will offer new insight in the mechanisms of cell sheet morphogenesis.
HHS’ Tracking Accountability in Government Grants System (TAGGS) website provides access to detailed descriptions of grants, loans, aggregated direct payments and other types of financial assistance awarded by the HHS Operating Divisions (OPDIVs) and the Office of the Secretary Staff Divisions (STAFFDIVs).
