Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( 🔒 ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Need Help

Wound Fluid as Regulator for Extracellular Matrix Remodeling and Recurrence in Inflammatory Breast Cancer

Award Number: R21TW010952
ORGANIZATION: FOGARTY INTERNATIONAL CENTER
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2017
PERIOD OF PERFORMANCE END DATE: 06/30/2021

Group Awards By:

View Award Description

Wound Fluid as Regulator for Extracellular Matrix Remodeling and Recurrence in Inflammatory Breast Cancer - It is well established that patients diagnosed with highly metastatic inflammatory breast cancer (IBC) have disease characterized by invasion of carcinoma cells into the lymphatic system, skin and axilla. High local recurrence rates on the chest wall and in the un-dissected regional nodes after neoadjuvant chemotherapy, mastectomy and radiation are well-documented and attributed to persistence of treatment resistant cells in the skin and residual lymphatics. Numerous genomic studies including recently presented next-generation sequencing demonstrate significant overlap with non-IBC cases suggesting non-genomic factors may influence the unique features of IBC. Together with studies showing that explicitly show non-tumor cells influence the IBC specific features of the tumor cells led us to investigate non-tumor factors to identify important targets in the microenvironment in IBC. Wound fluid obtained from post-surgical tumor cavities in non-IBC has recently been suggested to modulate biological activities in the tumor bed. In the present proposal, we aim to characterize cellular and proteomic components of the wound healing fluid collected during: 1) hemostasis/inflammatory; and 2) proliferating/tissue remodeling phases from non-IBC and IBC patients undergoing breast mastectomy. Wound fluid collected from benign breast lesions and mammoplasty will be used as control. First, we will collect the wound healing fluid to characterize epithelial and immunological cellular components using flow cytometric analysis. The proteomic profile of different phases of wound healing fluid will be assessed using cytokine antibody array. Since dynamic remodeling of the extracellular matrix (ECM) plays crucial role in cancer recurrence and dissemination, we will then develop 3D organotypic mammosphere (OMS) in-vitro culture model derived from patients’ carcinoma tissues to test how wound healing fluid may regulate ECM remodeling. OMS will be seeded in media conditioned by wound fluid collected at different phases of the wound healing. After appropriate time of incubation, OMS will be subjected to RT² Profiler PCR Array that simultaneously detects the expression of 84 genes involved in ECM remodeling and cell adhesion. Finally, we plan to validate significant candidates identified from these steps in independent data at MD Anderson Cancer Center. Our study is critically important as the low survival rates of breast cancer in Egypt, especially among IBC patients, indicates a pressing need to develop collaborative research programs that can be of immediate assistance to patients. We anticipate that our findings will identify biological factors in the IBC wound fluid that may contribute to the dissemination of carcinoma cells all over the body and make the disease resistant to radiation and chemotherapy. The proposed research will be conducted primarily in Cairo, Egypt by faculty at Cairo University and Ain Shams University with collaborative engagement and support from colleagues at MD Anderson Cancer Center in Houston, Texas and University of Maryland School of Medicine in Baltimore, Maryland.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = -$1,278 )
2022	2018	UNIVERSITY OF MARYLAND	220 ARCH ST RM 02148	BALTIMORE	MD	21201	BALTIMORE CITY	USA	International Research and Research Training	000	2	11/6/2021	NON-COMPETING CONTINUATION	-$1,278
														Subtotal = -$1,278

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2018	UNIVERSITY OF MARYLAND	220 ARCH ST RM 02148	BALTIMORE	MD	21201	BALTIMORE CITY	USA	International Research and Research Training	000	2	11/13/2020	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $155,062 )
2018	2018	UNIVERSITY OF MARYLAND	220 ARCH ST RM 02148	BALTIMORE	MD	21201	BALTIMORE CITY	USA	International Research and Research Training	000	2	7/20/2018	NON-COMPETING CONTINUATION	$155,062
														Subtotal = $155,062

Issue Date FY: 2017 ( Subtotal = $166,998 )
2017	2017	UNIVERSITY OF MARYLAND	220 ARCH ST RM 02148	BALTIMORE	MD	21201	BALTIMORE CITY	USA	International Research and Research Training	000	1	9/20/2017	NEW	$166,998
														Subtotal = $166,998

Grand Total All Awards = $320,782

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Wound Fluid as Regulator for Extracellular Matrix Remodeling and Recurrence in Inflammatory Breast Cancer

Award Number: R21TW010952

ORGANIZATION: FOGARTY INTERNATIONAL CENTER

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2017

PERIOD OF PERFORMANCE END DATE: 06/30/2021

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer