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Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imaging

Award Number: R21GM141675
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2021
PERIOD OF PERFORMANCE END DATE: 06/30/2025

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Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imaging - PROJECT SUMMARY Super-resolution imaging has been a revolutionary technique for biomedical sciences and provided invaluable information about cellular architecture and function at the molecular level with nanometer precision. However, the multiplexing capability and spatial resolution are mainly constrained by the performance of existing fluorescent labels. We propose to develop a palette of bright and switchable fluorescent dyes, referred to as SwitFluor, for multiplexing super-resolution imaging method to simultaneously resolve 6 molecular targets at 10-nm spatial resolution within 10 minutes. We aim to concurrently (1) achieve ultrahigh photon emission rate during the bright state of single-molecule switching process using a newly designed enhancer strand in DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) dye system, and (2) simultaneously switch fluorescence of 6 specially designed spectrally-distinct SwitFluor dyes using orthogonal imager strands for simultaneous imaging with our recently developed spectroscopic single-molecule localization microscopy (sSMLM) optical system. The integration of bright SwitFluor (DNA-PAINT) dyes with sSMLM will collectively achieve the proposed goal. The proposed technology could make a profound impact on a wide range of biological research as we will be able to study complex cellular processes deep down to the molecular scale.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2022	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	000	3	1/14/2026	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2022	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27695	WAKE	USA	Biomedical Research and Research Training	000	3	5/20/2024	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = -$79,199 )
2023	2022	NORTHWESTERN UNIVERSITY	633 CLARK STREET	EVANSTON	IL	60208	COOK	USA	Biomedical Research and Research Training	000	2	3/28/2023	NON-COMPETING CONTINUATION	-$239,999
2023	2022	NORTHWESTERN UNIVERSITY	633 CLARK STREET	EVANSTON	IL	60208	COOK	USA	Biomedical Research and Research Training	003	2	5/15/2023	NON-COMPETING CONTINUATION	-$1
2023	2022	NORTH CAROLINA STATE UNIVERSITY	2601 WOLF VILLAGE WAY	RALEIGH	NC	27607	WAKE	USA	Biomedical Research and Research Training	001	3	3/29/2023	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$199,973
2023	2021	NORTHWESTERN UNIVERSITY	633 CLARK STREET	EVANSTON	IL	60208	COOK	USA	Biomedical Research and Research Training	002	1	5/15/2023	NEW	-$39,172
														Subtotal = -$79,199

Issue Date FY: 2022 ( Subtotal = $240,000 )
2022	2022	NORTHWESTERN UNIVERSITY	633 CLARK	EVANSTON	IL	60208	COOK	USA	Biomedical Research and Research Training	000	2	8/18/2022	NON-COMPETING CONTINUATION	$240,000
														Subtotal = $240,000

Issue Date FY: 2021 ( Subtotal = $199,583 )
2021	2021	NORTHWESTERN UNIVERSITY	633 CLARK	EVANSTON	IL	60208	COOK	USA	Biomedical Research and Research Training	000	1	6/28/2021	NEW	$199,583
														Subtotal = $199,583

Grand Total All Awards = $360,384

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Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imaging

Award Number: R21GM141675

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2021

PERIOD OF PERFORMANCE END DATE: 06/30/2025

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