Monday, February 16, 2026 2/16/2026

Epigenetic regulation of hematopoiesis

Award Number: R01HL144712
ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/15/2019
PERIOD OF PERFORMANCE END DATE: 12/31/2023

Group Awards By:

View Award Description

Epigenetic regulation of hematopoiesis - Project Summary/Abstract Histone deacetylases (HDACs) are a group of epigenetic enzymes that are important in regulating gene transcription and other cellular processes. HDAC1 is implicated in various developmental program, including hematopoiesis. We and others previously showed that HDAC1 is an important regulator for GATA-1 function in FOG-1 dependent manner. During erythroid differentiation, HDAC1 within FOG-1 associated NuRD complex is acetylated and becomes inactive. Inactivated HDAC1 converts the NuRD into a coactivator complex to activate GATA-1 dependent gene transcription. Here we show that GATA-1 can also associate with HDAC1 in a FOG-1 independent manner, importantly, this association is required for GATA-1 deacetylation, therefore suggesting another layer of regulation for GATA-1 function. The significance of these findings is demonstrated by our observation that mutations in GATA-1 that disrupt HDAC1 interactions (GATA-1 2RA mutants) cause erythroid differentiation defects in mice. The central hypothesis for this proposal is that GATA-1 and HDAC1 interaction as well as acetylation of GATA-1 regulates the erythroid differentiation program and erythroid enhancer/promoter chromatin structure during erythropoiesis. In this proposal, we will investigate the mechanism underlying the regulation of GATA-1 activity by HDAC1 in a FOG-1 independent manner and to study the importance of direct HDAC1 binding in GATA-1 mediated gene transcription program and global GATA-1 recruitment and chromatin accessibility. Finally we propose to study the role of HDAC1 in regulating erythroid lineage differentiation by direct interacting with GATA-1 in a mouse model. By completion of this proposal, we will be able to gain insight into the function of dynamic GATA-1 acetylation/deacetylation and the importance of GATA-1/HDAC1 direct interactions. We will also learn the differential requirement of FOG-1 dependent and independent HDAC1 recruitment for erythropoiesis. The Knock in mouse work will further enable us to delineate the requirement of GATA-1/HDAC1 interactions for lineage specific development. Thus, this study will lead to in depth understanding of GATA-1 function in erythropoiesis and beyond.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2022	PENNSYLVANIA STATE UNIVERSITY	500 UNIVERSITY DRIVE	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	000	4	5/13/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $395,800 )
2022	2022	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY AVE RM C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	000	4	12/8/2021	NON-COMPETING CONTINUATION	$356,220
2022	2022	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY AVE RM C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	001	4	6/13/2022	NON-COMPETING CONTINUATION	$39,580
														Subtotal = $395,800

Issue Date FY: 2021 ( Subtotal = $395,800 )
2021	2021	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY AVE RM C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	003	3	5/24/2021	NON-COMPETING CONTINUATION	$39,580
2021	2021	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY AVE RM C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	000	3	12/7/2020	NON-COMPETING CONTINUATION	$356,220
2021	2020	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY AVE RM C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	002	2	12/10/2020	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$24,735
2021	2019	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Blood Diseases and Resources Research	001	1	12/10/2020	NEW	-$24,735
														Subtotal = $395,800

Issue Date FY: 2020 ( Subtotal = $395,800 )
2020	2020	PENNSYLVANIA STATE UNIVERSITY, THE	500 UNIVERSITY DR C1607	HERSHEY	PA	17033	DAUPHIN	USA	Blood Diseases and Resources Research	001	2	4/24/2020	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$395,800
2020	2019	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Blood Diseases and Resources Research	000	1	4/23/2020	NEW	$0
														Subtotal = $395,800

Issue Date FY: 2019 ( Subtotal = $378,423 )
2019	2019	UNIVERSITY OF FLORIDA	1523 UNION RD RM 207	GAINESVILLE	FL	32611	ALACHUA	USA	Blood Diseases and Resources Research	000	1	1/7/2019	NEW	$378,423
														Subtotal = $378,423

Grand Total All Awards = $1,565,823

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Epigenetic regulation of hematopoiesis

Award Number: R01HL144712

ORGANIZATION: NATIONAL HEART, LUNG, & BLOOD INSTITUTE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/15/2019

PERIOD OF PERFORMANCE END DATE: 12/31/2023

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer