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New techniques for detecting and handling nanocrystals for cutting edge structural biology methods

Award Number: R01GM141273
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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New techniques for detecting and handling nanocrystals for cutting edge structural biology methods - Project Summary Determining the detailed structural characteristics of biomolecules relevant to human health and disease is one of the most crucial tools in our arsenal for understanding disease etiology and mechanism, and for being able to develop new therapeutics that target these molecular entities. There are new techniques in structural biology, including serial femtosecond crystallography, serial synchrotron crystallography, and microcrystal electron diffraction, that have the potential to greatly advance structure determination of biomolecules and to empower access to structural details that have deﬁed characterization via other structural methods. These new structural methods all rely on being able to generate, detect and appropriately handle extremely small crystalline samples of biomolecules. This requirement for sub- micron sized crystals is one of the key features of these technologies, and presents a major obstacle to the advancement of these methods for structure determination. This proposal presents innovative technologies for both image analysis and sample handling expressly designed to address the speciﬁc challenges of working with submicron crystals. We plan to use nonlinear optical microscopy methods coupled with purpose-built application of point process modeling and wavelet image analysis approaches to provide computational tools needed to enable detection and characterization of submicron samples that are invisible to the brightﬁeld microscopy tools that are typically used in sample generation and experimental set up for crystal based structural biology. In addition, we will examine different ﬁxed target platforms to reduce sample handling, minimizing potential crystal damage, as well as test use of acoustic droplet ejection techniques for nanoliter volume sample transfer. These innovations will be a powerful addition to structural biology toolbox for leveraging the cutting edge diffraction based methods currently available for structure determination. These technology developments will break through key barriers to the widespread use of these cutting edge structural methods.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $388,000 )
2024	2024	HAUPTMAN WOODWARD MEDICAL RESEARCH INSTITUTE, INC	700 ELLICOTT ST # 700	BUFFALO	NY	14203	ERIE	USA	Biomedical Research and Research Training	000	3	8/19/2024	NON-COMPETING CONTINUATION	$388,000
														Subtotal = $388,000

Issue Date FY: 2023 ( Subtotal = $388,000 )
2023	2023	HAUPTMAN WOODWARD MEDICAL RESEARCH INSTITUTE, INC.	700 ELLICOTT ST	BUFFALO	NY	14203	ERIE	USA	Biomedical Research and Research Training	000	2	8/28/2023	NON-COMPETING CONTINUATION	$388,000
														Subtotal = $388,000

Issue Date FY: 2022 ( Subtotal = $407,732 )
2022	2022	HAUPTMAN WOODWARD MEDICAL RESEARCH INSTITUTE, INC.	700 ELLICOTT ST	BUFFALO	NY	14203	ERIE	USA	Biomedical Research and Research Training	000	1	9/16/2022	NEW	$407,732
														Subtotal = $407,732

Grand Total All Awards = $1,183,732

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New techniques for detecting and handling nanocrystals for cutting edge structural biology methods

Award Number: R01GM141273

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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