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Molecular and cellular biology of the phage nucleus and spindle

Award Number: R01GM129245
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/06/2018
PERIOD OF PERFORMANCE END DATE: 07/31/2026

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Molecular and cellular biology of the phage nucleus and spindle - Project Summary The ability to establish and maintain subcellular organization is a fundamental design principle of biological systems. We recently discovered that many jumbo phage establish surprisingly complex subcellular organization involving a nucleus-like structure or “phage nucleus” that compartmentalizes phage replication within the host cytoplasm. The phage nucleus carries out many functions previously only attributed to the eukaryotic nucleus including segregation of key processes (DNA replication and repair, mRNA synthesis, protein synthesis and metabolism) and selective protein import and mRNA export. However, the phage nucleus is structurally dissimilar to the eukaryotic nucleus, being mainly composed of a single layer of a phage-encoded protein and lacking evident openings for exchange with the cytoplasm instead of a double-layer lipid membrane containing prominent pore complexes. We have identified the key protein that makes up the phage nucleus shell; however, the components required to assemble a fully functional phage nucleus and their roles in this multipurpose structure remain unknown. Here we aim to identify and characterize additional proteins that are required to construct a fully functional phage nucleus to gain greater insight into the biology of these understudied viruses and the underlying principles of subcellular compartmentalization previously observed almost exclusively in eukaryotes. We will use an integrative approach that combines genetics, cell biology, biochemistry and in situ and in vitro structural biology approaches to study the jumbo phage in Pseudomonas and E. coli. Together, the knowledge gained from this project will provide insight into fundamental principles underlying subcellular organization.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $0 )
2026	2025	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	8	11/20/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2025 ( Subtotal = $493,176 )
2025	2025	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	8	8/20/2025	NON-COMPETING CONTINUATION	$493,176
														Subtotal = $493,176

Issue Date FY: 2024 ( Subtotal = $743,176 )
2024	2024	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	002	7	9/10/2024	SUPPLEMENT FOR EXPANSION	$250,000
2024	2024	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	001	7	8/16/2024	NON-COMPETING CONTINUATION	$493,176
2024	2023	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	6	7/30/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $743,176

Issue Date FY: 2023 ( Subtotal = $493,176 )
2023	2023	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	002	6	8/28/2023	NON-COMPETING CONTINUATION	$493,176
2023	2022	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	001	5	3/24/2023	COMPETING CONTINUATION	$115,190
2023	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	4	3/24/2023	NON-COMPETING CONTINUATION	-$115,190
														Subtotal = $493,176

Issue Date FY: 2022 ( Subtotal = $493,176 )
2022	2022	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	001	5	9/26/2022	COMPETING CONTINUATION	$493,176
2022	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	4	9/26/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $493,176

Issue Date FY: 2021 ( Subtotal = $490,897 )
2021	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	4	8/2/2021	NON-COMPETING CONTINUATION	$490,897
														Subtotal = $490,897

Issue Date FY: 2020 ( Subtotal = $489,819 )
2020	2020	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	3	7/15/2020	NON-COMPETING CONTINUATION	$489,819
														Subtotal = $489,819

Issue Date FY: 2019 ( Subtotal = $490,016 )
2019	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	2	7/29/2019	NON-COMPETING CONTINUATION	$490,016
														Subtotal = $490,016

Issue Date FY: 2018 ( Subtotal = $488,942 )
2018	2018	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	9/6/2018	NEW	$488,942
														Subtotal = $488,942

Grand Total All Awards = $4,182,378

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Molecular and cellular biology of the phage nucleus and spindle

Award Number: R01GM129245

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/06/2018

PERIOD OF PERFORMANCE END DATE: 07/31/2026

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