Sunday, July 6, 2025 7/6/2025

Biological Spectroscopy and Crystallography Using an X-ray Free Electron Laser

Award Number: R01GM110501
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2014
PERIOD OF PERFORMANCE END DATE: 02/28/2027

Group Awards By:

View Award Description

Biological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Project Summary/Abstract The goal of this proposal is to understand nature’s well-controlled chemistry that occurs in metalloenzymes, by developing the necessary tools and applying them to follow the structural dynamics of the protein and chemical dynamics of the metal-catalyst. For this purpose, we will use X-ray crystallography and X-ray spectroscopy techniques at X-ray Free Electron Lasers (XFELs). Although the structure of enzymes and the chemistry at the catalytic sites have been studied intensively, an understanding of the atomic-scale chemistry requires a new approach beyond the conventional steady state X-ray crystallography and X-ray spectroscopy at cryogenic temperatures. Following the dynamic changes in the geometric and electronic structure of metalloenzymes at ambient temperature, while overcoming the severe X-ray damage to the redox active catalytic center, is key for deriving the reaction mechanism. The intense and ultra-short femtosecond (fs) X-ray pulses from XFELs provide an opportunity to overcome the current limitations of room temperature data collection for biological samples at synchrotron X- ray sources. The fs X-ray pulses with shot-by-shot sample replacement make it possible to acquire the signal before damage occurs and the sample is destroyed. We will design and apply a suite of time-resolved X-ray diffraction and X-ray absorption/emission spectroscopy methods, that make use of the unique properties of the XFEL beam. This will provide an unprecedented combination of correlated data between the protein and the co-factors, all of which are necessary to understand the interplay between the geometric structure of the protein and electronic structure of the metal complex, and the functional consequences. Spectroscopy, both emission and absorption spectroscopy, will provide the time-evolution of the electronic structure, while simultaneous room temperature X-ray crystallography will help us visualize the changes in the geometric structure of the overall protein. We will use these methodologies to study some of the most important metalloenzymes in biology to gain insights into the catalytic mechanisms, including mono, and dinuclear systems both with homo- and hetero-metallic centers. A representative example of these systems are Fe enzymes for oxygen and C-H bond activation where the involvement of high-valent Fe are proposed, such as the heme containing Cyt P450 systems, non-heme enzymes ribonucleotide reductase (Mn/Fe and Fe/Fe), and methane mono oxygenase (Fe/Fe). We will also focus on Ni and Ni/Fe enzymes relevant to H2 generation and methane metabolism, and functional analogs of the important class of heme-copper oxidase systems engineered into simpler proteins.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $392,287 )
2025	2025	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	11	4/15/2025	NON-COMPETING CONTINUATION	$392,287
														Subtotal = $392,287

Issue Date FY: 2024 ( Subtotal = $392,287 )
2024	2024	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	003	10	6/17/2024	NON-COMPETING CONTINUATION	$39,056
2024	2024	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	002	10	3/8/2024	NON-COMPETING CONTINUATION	$353,231
2024	2023	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	001	9	3/1/2024	COMPETING CONTINUATION	$105,169
2024	2021	THE REGENTS OF THE UNIVERSITY OF CALIFORNIA	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	8	3/1/2024	NON-COMPETING CONTINUATION	-$105,169
														Subtotal = $392,287

Issue Date FY: 2023 ( Subtotal = $541,304 )
2023	2023	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	001	9	2/24/2023	COMPETING CONTINUATION	$541,304
2023	2021	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	8	2/15/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $541,304

Issue Date FY: 2021 ( Subtotal = $379,146 )
2021	2021	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	8	8/31/2021	NON-COMPETING CONTINUATION	$379,146
														Subtotal = $379,146

Issue Date FY: 2020 ( Subtotal = $379,146 )
2020	2020	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	7	8/24/2020	NON-COMPETING CONTINUATION	$379,146
														Subtotal = $379,146

Issue Date FY: 2019 ( Subtotal = $379,620 )
2019	2019	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	003	6	9/5/2019	NON-COMPETING CONTINUATION	$379,620
2019	2018	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	002	5	4/24/2019	COMPETING CONTINUATION	$46,564
2019	2018	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	5	11/16/2018	COMPETING CONTINUATION	$0
2019	2017	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	001	4	4/24/2019	NON-COMPETING CONTINUATION	-$46,564
														Subtotal = $379,620

Issue Date FY: 2018 ( Subtotal = $388,857 )
2018	2018	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	5	9/21/2018	COMPETING CONTINUATION	$337,115
2018	2018	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	002	5	9/25/2018	COMPETING CONTINUATION	$51,742
2018	2017	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	001	4	9/22/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $388,857

Issue Date FY: 2017 ( Subtotal = $321,044 )
2017	2017	REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE	1 CYCLOTRON RD	BERKELEY	CA	94720	ALAMEDA	USA	Biomedical Research and Research Training	000	4	8/15/2017	NON-COMPETING CONTINUATION	$321,044
														Subtotal = $321,044

Issue Date FY: 2016 ( Subtotal = $353,098 )
2016	2016	LAWRENCE BERKELEY LABORATORY	POST OFFICE BOX 528	BERKELEY	CA	94701	ALAMEDA	USA	Biomedical Research and Research Training	000	3	8/31/2016	NON-COMPETING CONTINUATION	$353,098
														Subtotal = $353,098

Issue Date FY: 2015 ( Subtotal = $507,880 )
2015	2015	LAWRENCE BERKELEY LABORATORY	POST OFFICE BOX 528	BERKELEY	CA	94701	ALAMEDA	USA	Biomedical Research and Research Training	000	2	8/25/2015	NON-COMPETING CONTINUATION	$385,630
2015	2015	LAWRENCE BERKELEY LABORATORY	POST OFFICE BOX 528	BERKELEY	CA	94701	ALAMEDA	USA	Biomedical Research and Research Training	002	2	9/22/2015	SUPPLEMENT FOR EXPANSION	$22,250
2015	2015	LAWRENCE BERKELEY LABORATORY	POST OFFICE BOX 528	BERKELEY	CA	94701	ALAMEDA	USA	Biomedical Research and Research Training	001	2	8/28/2015	SUPPLEMENT FOR EXPANSION	$100,000
														Subtotal = $507,880

Issue Date FY: 2014 ( Subtotal = $383,776 )
2014	2014	LAWRENCE BERKELEY LABORATORY	POST OFFICE BOX 528	BERKELEY	CA	94701	ALAMEDA	USA	Biomedical Research and Research Training	000	1	9/11/2014	NEW	$383,776
														Subtotal = $383,776

Grand Total All Awards = $4,418,445

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Biological Spectroscopy and Crystallography Using an X-ray Free Electron Laser

Award Number: R01GM110501

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2014

PERIOD OF PERFORMANCE END DATE: 02/28/2027

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer