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Dynamics of Clathrin Coat Formation in Cells

Award Number: R01GM075252
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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DESCRIPTION (provided by applicant): Cells require ordered movement of proteins and lipids from one membrane-bound compartment to another, while maintaining the organization, function and heterogeneity of the donor and acceptor membranes; vesicular membrane traffic carries out these functions. Clathrin coated vesicles are the most prominent form of traffic from the plasma membrane to endosomes (endocytosis), a pathway by which various ligands and their receptors enter cells. Clathrin coated vesicles are also important for traffic between endosomes and the trans-Golgi network (TGN). We propose to continue our studies of molecular mechanisms in clathrin-mediated membrane traffic, using direct observation by live-cell imaging with single-molecular sensitivity and high spatial and temporal resolution, to answer the following questions. (1) What are the molecular events required to initiate formation of a clathrin coated pit? (2) What are the molecular components of coats trapped at various stages of growth and closure: early and late abortive coated pits, and pits stalled at a relatively late stage, by imposing tension of the membrane and by inhibiting actin dynamics? (3) What is the mechanism of clathrin uncoating mediated by Hsc70 and its co-chaperone auxilin and how is the uncoating coordinated with assembly so that the two processes do not compete?


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2018	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	13	7/12/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $611,770 )
2018	2018	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	002	13	8/22/2018	SUPPLEMENT FOR EXPANSION	$207,113
2018	2018	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	13	6/25/2018	NON-COMPETING CONTINUATION	$40,466
2018	2018	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	13	12/2/2017	NON-COMPETING CONTINUATION	$364,191
														Subtotal = $611,770

Issue Date FY: 2017 ( Subtotal = $404,657 )
2017	2017	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	12	6/20/2017	NON-COMPETING CONTINUATION	$40,466
2017	2017	CHILDREN'S HOSPITAL CORPORATION, THE	300 LONGWOOD AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	12	12/6/2016	NON-COMPETING CONTINUATION	$364,191
														Subtotal = $404,657

Issue Date FY: 2016 ( Subtotal = $404,657 )
2016	2016	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	11	12/3/2015	NON-COMPETING CONTINUATION	$364,191
2016	2016	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	11	4/6/2016	NON-COMPETING CONTINUATION	$40,466
														Subtotal = $404,657

Issue Date FY: 2015 ( Subtotal = $402,942 )
2015	2015	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	10	1/12/2015	COMPETING CONTINUATION	$402,942
2015	2014	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	9	1/7/2015	NON-COMPETING CONTINUATION	$0
														Subtotal = $402,942

Issue Date FY: 2014 ( Subtotal = $390,000 )
2014	2014	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	9	11/22/2013	NON-COMPETING CONTINUATION	$351,000
2014	2014	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	9	3/25/2014	NON-COMPETING CONTINUATION	$39,000
														Subtotal = $390,000

Issue Date FY: 2013 ( Subtotal = $376,350 )
2013	2013	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	004	8	6/17/2013	NON-COMPETING CONTINUATION	$25,350
2013	2013	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	003	8	12/10/2012	NON-COMPETING CONTINUATION	$351,000
2013	2012	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	7	11/21/2012	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$77,040
2013	2012	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	6	11/21/2012	NON-COMPETING CONTINUATION	-$77,040
2013	2012	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	002	7	12/6/2012	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $376,350

Issue Date FY: 2012 ( Subtotal = $420,000 )
2012	2012	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	6	3/19/2012	NON-COMPETING CONTINUATION	$42,000
2012	2012	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	6	12/2/2011	NON-COMPETING CONTINUATION	$378,000
														Subtotal = $420,000

Issue Date FY: 2011 ( Subtotal = $420,000 )
2011	2011	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	5	12/13/2010	COMPETING CONTINUATION	$420,000
														Subtotal = $420,000

Issue Date FY: 2008 ( Subtotal = $287,337 )
2008	2008	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	4	8/15/2008	NON-COMPETING CONTINUATION	$287,337
														Subtotal = $287,337

Issue Date FY: 2007 ( Subtotal = $287,337 )
2007	2007	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	3	8/17/2007	NON-COMPETING CONTINUATION	$287,337
														Subtotal = $287,337

Issue Date FY: 2006 ( Subtotal = $295,919 )
2006	2006	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	2	8/29/2006	NON-COMPETING CONTINUATION	$295,919
														Subtotal = $295,919

Issue Date FY: 2005 ( Subtotal = $303,040 )
2005	2005	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	1	9/5/2005	NEW	$303,040
														Subtotal = $303,040

Grand Total All Awards = $4,604,009

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Dynamics of Clathrin Coat Formation in Cells

Award Number: R01GM075252

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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