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DYNAMICS IN LIVE CELLS BY FICS

Award Number: R01GM067891
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: HEALTH SERVICES
PERIOD OF PERFORMANCE START DATE: 05/01/2003
PERIOD OF PERFORMANCE END DATE: 04/30/2007

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DYNAMICS IN LIVE CELLS BY FICS - DESCRIPTION (provided by applicant): The overall goal of this research is to further develop, test and implement our original experimental technique to study dynamic processes in living cells. Because the cytosol is a densely crowded and dynamically heterogeneous environment, measurements that establish a connection between spatial scale and mobility can provide insight into its organization and mechanisms of cytoskeletal activity. Fourier imaging correlation spectroscopy (FICS) is an attemative to direct imaging methods and is based on selectively detecting fluorescence fluctuations from N labeled sub-cellular species at a specified (and adjustable) spatial scale. Our recent FICS studies of the mitochondriat reticulum in live osteosarcoma cells show that the length-scale-dependence of the organelle's mobility follows a systematic and complex pattern, which likely reflects the relevant length- and time-scales of cytoskeletal motor protein activity. When actin or tubuiin components of the cytoskeleton are selectively removed, the organelle's dynamics become sequentially simplified, in the absence of both cytosketal components, the dynamics of the reticulum become primarily diffusive and may be modelled as a collection of friction points interconnected by elastic springs. The main advantage of FICS is that the signal is enhanced (relative to that from a single particle) by a factor of N and thereby affords superior temporal resolution and signal sensitivity in comparison to fluorescence microscopy. In this proposal, we develop Molecular FICS, a variation of the method that is sensitive to the motions and structural changes of individual proteins and other macromolecules in the celt. The combination of Molecular FICS with the previously established organelle-sensitive version of the method will allow us to study how macromolecular species are transported through their intracellular compartments.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2006 ( Subtotal = $238,628 )
2006	2006	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	4	5/1/2006	NON-COMPETING CONTINUATION	$238,628
														Subtotal = $238,628

Issue Date FY: 2005 ( Subtotal = $245,441 )
2005	2005	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	3	4/29/2005	NON-COMPETING CONTINUATION	$245,441
														Subtotal = $245,441

Issue Date FY: 2004 ( Subtotal = $246,443 )
2004	2004	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	2	4/29/2004	NON-COMPETING CONTINUATION	$246,443
														Subtotal = $246,443

Issue Date FY: 2003 ( Subtotal = $297,379 )
2003	2003	UNIVERSITY OF OREGON	5215 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Cell Biology and Biophysics Research	000	1		NEW	$297,379
														Subtotal = $297,379

Grand Total All Awards = $1,027,891

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DYNAMICS IN LIVE CELLS BY FICS

Award Number: R01GM067891

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: HEALTH SERVICES

PERIOD OF PERFORMANCE START DATE: 05/01/2003

PERIOD OF PERFORMANCE END DATE: 04/30/2007

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