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Mitotic Spindle Assembly & Function in C. elegans - Equipment Supplement

Award Number: R01GM049869
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Project Summary/Abstract We request funds to purchase of a second CherryTemp device, produced by a company in France called Cherry Biotech, that provides precise temperature control of specimens mounted on slides during live cell imaging, and allows for very rapid changes in sample temperature (see Narrative for details). In the Specific Aims for our most recent renewal of R01GM049869, and in our research program outlined in R35GM131749, we proposed to use temperature-sensitive, embryonic-lethal (TS-EL) C. elegans mutants to investigate the genetic pathways that mediate the acentrosomal assembly of bipolar spindles during oocyte meiotic cell division, relying in large part on live-imaging of TS-EL mutants to define gene requirements. The temperature control provided by the CherryTemp device has greatly improve our ability to accurately and reproducibly assess gene requirements using our TS-EL mutants. Our Institute recently obtain funds from the Murdock Foundation to purchase a second Nikon spinning disk confocal microscope. This second CherryTemp device we now wish to purchase will come with components that allow it to be used on both our current Andor system and the new Nikon spinning disk confocal system. This new device will serve both as a backup for our current device when it breaks down (which it has at times, with one thermostat that is sensitive to wear and tear), and more importantly will allow my lab members to use both our Andor System and the new Nikon system simultaneously, with one lab member working on each scope, doubling our rate of data acquisition. Thus these devices not only will improve the rigor and reproducibility of our data, but also greatly accelerate our rate of data collection and how quickly we can achieve our research goals and initiate new projects.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2019	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	24	2/14/2021	SUPPLEMENT FOR EXPANSION	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $27,529 )
2019	2019	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	002	24	7/31/2019	SUPPLEMENT FOR EXPANSION	$27,529
2019	2018	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	24	7/19/2019	NON-COMPETING CONTINUATION	$0
2019	2018	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	24	4/22/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $27,529

Issue Date FY: 2018 ( Subtotal = $483,020 )
2018	2018	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	24	8/31/2018	NON-COMPETING CONTINUATION	$483,020
														Subtotal = $483,020

Issue Date FY: 2017 ( Subtotal = $506,093 )
2017	2017	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	004	23	8/15/2017	NON-COMPETING CONTINUATION	$483,020
2017	2017	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	005	23	9/5/2017	SUPPLEMENT FOR EXPANSION	$23,073
2017	2016	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	003	22	4/14/2017	COMPETING CONTINUATION	$10,332
2017	2016	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	22	1/21/2017	COMPETING CONTINUATION	$36,947
2017	2015	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	002	21	4/14/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$10,332
2017	2015	UNIVERSITY OF OREGON	1585 E 13TH AVE	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	21	1/21/2017	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	-$36,947
														Subtotal = $506,093

Issue Date FY: 2016 ( Subtotal = $458,786 )
2016	2016	UNIVERSITY OF OREGON	POST OFFICE BOX 3237	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	22	8/4/2016	COMPETING CONTINUATION	$458,786
2016	2015	UNIVERSITY OF OREGON	POST OFFICE BOX 3237	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	21	8/1/2016	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$0
														Subtotal = $458,786

Issue Date FY: 2015 ( Subtotal = $448,113 )
2015	2015	UNIVERSITY OF OREGON	POST OFFICE BOX 3237	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	21	7/16/2015	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$448,113
														Subtotal = $448,113

Issue Date FY: 2014 ( Subtotal = $447,904 )
2014	2014	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	20	7/21/2014	NON-COMPETING CONTINUATION	$448,591
2014	2013	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	19	11/7/2013	SUPPLEMENT FOR EXPANSION	-$687
														Subtotal = $447,904

Issue Date FY: 2013 ( Subtotal = $428,066 )
2013	2013	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	19	8/29/2013	SUPPLEMENT FOR EXPANSION	$100,375
2013	2013	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	002	19	11/7/2013	SUPPLEMENT FOR EXPANSION	-$687
2013	2013	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	19	7/24/2013	NON-COMPETING CONTINUATION	$328,378
														Subtotal = $428,066

Issue Date FY: 2012 ( Subtotal = $340,706 )
2012	2012	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	18	7/10/2012	COMPETING CONTINUATION	$340,706
2012	2010	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	17	7/9/2012	NON-COMPETING CONTINUATION	$0
														Subtotal = $340,706

Issue Date FY: 2010 ( Subtotal = $326,042 )
2010	2010	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	17	8/26/2010	NON-COMPETING CONTINUATION	$326,042
														Subtotal = $326,042

Issue Date FY: 2009 ( Subtotal = $330,058 )
2009	2009	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	16	8/13/2009	NON-COMPETING CONTINUATION	$330,058
														Subtotal = $330,058

Issue Date FY: 2008 ( Subtotal = $289,897 )
2008	2008	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	15	8/27/2008	NON-COMPETING CONTINUATION	$289,897
														Subtotal = $289,897

Issue Date FY: 2007 ( Subtotal = $331,367 )
2007	2007	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	14	9/27/2007	COMPETING CONTINUATION	$331,367
														Subtotal = $331,367

Issue Date FY: 2006 ( Subtotal = $402,209 )
2006	2006	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	13	4/17/2006	NON-COMPETING CONTINUATION	$402,209
														Subtotal = $402,209

Issue Date FY: 2005 ( Subtotal = $409,310 )
2005	2005	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	001	12	6/13/2005	SUPPLEMENT FOR EXPANSION	$124,394
2005	2005	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	12	3/18/2005	NON-COMPETING CONTINUATION	$284,916
														Subtotal = $409,310

Issue Date FY: 2004 ( Subtotal = $285,577 )
2004	2004	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Biomedical Research and Research Training	000	11	3/25/2004	NON-COMPETING CONTINUATION	$285,577
														Subtotal = $285,577

Issue Date FY: 2003 ( Subtotal = $286,195 )
2003	2003	UNIVERSITY OF OREGON	5215 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Genetics and Developmental Biology Research and Research Training	000	10		COMPETING CONTINUATION	$286,195
														Subtotal = $286,195

Issue Date FY: 2002 ( Subtotal = $238,609 )
2002	2002	UNIVERSITY OF OREGON	5215 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Genetics and Developmental Biology Research and Research Training	000	9	4/1/2002	NON-COMPETING CONTINUATION	$238,609
														Subtotal = $238,609

Issue Date FY: 2001 ( Subtotal = $231,894 ) (Continued on the next page)
Grand Total All Awards = $7,002,794

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Mitotic Spindle Assembly & Function in C. elegans - Equipment Supplement

Award Number: R01GM049869

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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