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Molecular Interactions in Clathrin Coated Vesicles

Award Number: R01GM036548
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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DESCRIPTION (provided by applicant): Clathrin coated vesicles are the most intensively studied carriers of membrane traffic in eukaryotic cells and the most prominent form of traffic from the plasma membrane to endosomes (endocytosis). Clathrin-coated pits and vesicles were the first membrane-traffic system recognized and analyzed in detail, and they have become a paradigm for efforts to understand molecular mechanisms of other modes of vesicular transport. We have recently combined high spatial-resolution snapshots from cryoEM with data from high temporal-resolution light microscopy (with single-molecule sensitivity) to answer one long-standing question about the way the clathrin pathway operates: how does the uncoating ATPase, Hsc70, together with its co-chaperone, auxilin, catalyze coat disassembly? Each of the Aims of this proposal seeks to exploit the single-molecule sensitivity of in vitro, single-particle methods as used in that work, to analyze mechanisms of general significance for intracellular membrane traffic. In Aim 1, we will determine the molecular mechanism by which auxilin detects membrane scission. In Aim 2, we will use in vitro reconstitution experiments to dissect the steps of coated pit initiation and growth. In Aim 3, we will study the molecular organization of Eps15 and its partners (epsin, intersection, FCH01/2) -- a complex, network-like assembly, with multiple cross-interacting components.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2014 ( Subtotal = $847,333 )
2014	2014	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	30	8/26/2014	SUPPLEMENT FOR EXPANSION	$131,133
2014	2014	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	30	6/20/2014	NON-COMPETING CONTINUATION	$716,200
														Subtotal = $847,333

Issue Date FY: 2013 ( Subtotal = $585,285 )
2013	2013	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	002	29	6/14/2013	NON-COMPETING CONTINUATION	$691,133
2013	2012	CHILDREN'S HOSPITAL CORPORATION	300 LONGWOOD AVENUE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	28	12/31/2012	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$707,921
2013	2012	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	27	12/31/2012	NON-COMPETING CONTINUATION	-$813,769
														Subtotal = $585,285

Issue Date FY: 2012 ( Subtotal = $813,769 )
2012	2012	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	27	6/29/2012	NON-COMPETING CONTINUATION	$813,769
														Subtotal = $813,769

Issue Date FY: 2011 ( Subtotal = $813,765 )
2011	2011	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	001	26	8/11/2011	COMPETING CONTINUATION	$813,765
2011	2010	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	25	8/10/2011	NON-COMPETING CONTINUATION	$0
														Subtotal = $813,765

Issue Date FY: 2010 ( Subtotal = $764,407 )
2010	2010	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	25	6/18/2010	NON-COMPETING CONTINUATION	$764,407
														Subtotal = $764,407

Issue Date FY: 2009 ( Subtotal = $749,639 )
2009	2009	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	24	7/1/2009	NON-COMPETING CONTINUATION	$749,639
														Subtotal = $749,639

Issue Date FY: 2008 ( Subtotal = $656,409 )
2008	2008	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	23	7/7/2008	NON-COMPETING CONTINUATION	$656,409
														Subtotal = $656,409

Issue Date FY: 2007 ( Subtotal = $656,324 )
2007	2007	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	22	6/18/2007	COMPETING CONTINUATION	$656,324
														Subtotal = $656,324

Issue Date FY: 2006 ( Subtotal = $595,397 )
2006	2006	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	21	6/12/2006	NON-COMPETING CONTINUATION	$595,397
														Subtotal = $595,397

Issue Date FY: 2005 ( Subtotal = $592,160 )
2005	2005	CBR INSTITUTE FOR BIOMEDICAL RESEARCH INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	20	6/27/2005	NON-COMPETING CONTINUATION	$592,160
														Subtotal = $592,160

Issue Date FY: 2004 ( Subtotal = $575,102 )
2004	2004	CENTER FOR BLOOD RESEARCH, INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Biomedical Research and Research Training	000	19	6/23/2004	NON-COMPETING CONTINUATION	$575,102
														Subtotal = $575,102

Issue Date FY: 2003 ( Subtotal = $558,544 )
2003	2003	CENTER FOR BLOOD RESEARCH, INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	18	6/30/2003	COMPETING CONTINUATION	$558,544
														Subtotal = $558,544

Issue Date FY: 2002 ( Subtotal = $534,252 )
2002	2002	CENTER FOR BLOOD RESEARCH, INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	17	12/24/2001	NON-COMPETING CONTINUATION	$534,252
														Subtotal = $534,252

Issue Date FY: 2001 ( Subtotal = $525,108 )
2001	2001	CENTER FOR BLOOD RESEARCH, INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	16	1/26/2001	NON-COMPETING CONTINUATION	$525,108
														Subtotal = $525,108

Issue Date FY: 2000 ( Subtotal = $516,229 )
2000	2000	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	15	12/6/1999	NON-COMPETING CONTINUATION	$516,229
														Subtotal = $516,229

Issue Date FY: 1999 ( Subtotal = $516,230 )
1999	1999	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	14	12/31/1998	COMPETING CONTINUATION	$516,230
														Subtotal = $516,230

Issue Date FY: 1998 ( Subtotal = $453,451 )
1998	1998	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	001	13	2/23/1998	SUPPLEMENT FOR EXPANSION	$95,794
1998	1998	IMMUNE DISEASE INSTITUTE INC	800 HUNTINGTON AVE	BOSTON	MA	02115	SUFFOLK	USA	Cell Biology and Biophysics Research	000	13	12/3/1997	NON-COMPETING CONTINUATION	$357,657
														Subtotal = $453,451

Grand Total All Awards = $10,753,404

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Molecular Interactions in Clathrin Coated Vesicles

Award Number: R01GM036548

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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