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Paternal DDT exposure and programming of metabolic dysfunction and cancer in offspring: Understanding the role of sperm mirnas and placenta development

Award Number: R01ES031611
ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Exposure to chemicals present in the environment can induce epigenetic changes in paternal sperm and affect risk of disease in offspring. This molecular memory of past exposures can be transmitted between generations via sperm non-coding RNAs such miRNAs. Our long-term goals to understand how parental environmental exposures can predispose children to diseases such as diabetes and cancer aligns with aims in the NIEHS’ strategic planning. The pesticide DDT(dichlorodiphenyltrichloroethane) is an environmental toxicant with endocrine disruptor (EDC) activity. While banned from Western countries for over 30 years, DDT is a persistent environmental pollutant that is still is detected in the American population, particularly in minorities and recent immigrants. Currently, the major of source of this pesticide in the U.S. is food imported from regions where DDT is used. Our preliminary data, generated in a mouse model, show that pre-conception exposure to DDT alters miRNAs in paternal sperm. More importantly, paternal DDT leads to low birth weight, a phenotype associated with reduced placenta and fetal size. Offspring of DDT fathers show metabolic dysfunction and accelerated cancer growth. We hypothesize that programming of offspring’s disease by pre-conception paternal DDT exposure occurs via sperm miRNA which alters placenta development and fetal growth. We also hypothesize that DDT exposure signals are relayed to sperm via extracellular vesicles secreted by epididymal cells. Our hypothesis will be tested in a mouse model and in cell cultures by focusing on the following aims: 1) To examine the mechanisms by which environmentally relevant doses of DDT and its metabolite, DDE, alter the miRNA (and other small RNAs) content in paternal sperm; 2) To characterize the mechanisms underlying alterations in placenta development and function resulting from paternal DDT exposure; 3) To evaluate whether miRNAs (and possibly other small RNAs) in sperm of DDT exposed males are mechanistically linked to alterations in placenta and fetal development. While the evidence showing that paternal exposures programs disease in offspring is robust, our understanding of the underlying mechanisms is still lacking. Defining the mechanisms by which paternal exposure to DDT and other EDCs can promote changes in fetal and placenta development is critical to identifying preventive tools for disease such as diabetes and cancer. This study will also contribute the general understanding of environmentally-induced non-genetic inheritance and could lead to public health recommendations to men of reproductive age. Finally, our findings could lead to potential placental biomarkers of parental exposure.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $499,214 )
2025	2025	GEORGETOWN UNIVERSITY	MAIN CAMPUS	WASHINGTON	DC	20057	DISTRICT OF COLUMBIA	USA	Environmental Health	000	5	11/20/2024	NON-COMPETING CONTINUATION	$499,214
														Subtotal = $499,214

Issue Date FY: 2024 ( Subtotal = $517,778 )
2024	2024	GEORGETOWN UNIVERSITY	MAIN CAMPUS	WASHINGTON	DC	20057	DISTRICT OF COLUMBIA	USA	Environmental Health	000	4	11/16/2023	NON-COMPETING CONTINUATION	$517,778
														Subtotal = $517,778

Issue Date FY: 2023 ( Subtotal = $544,298 )
2023	2023	GEORGETOWN UNIVERSITY	MAIN CAMPUS	WASHINGTON	DC	20057	DISTRICT OF COLUMBIA	USA	Environmental Health	000	3	12/2/2022	NON-COMPETING CONTINUATION	$544,298
														Subtotal = $544,298

Issue Date FY: 2022 ( Subtotal = $544,298 )
2022	2022	GEORGETOWN UNIVERSITY (THE)	37TH AND O ST NW	WASHINGTON	DC	20057	DISTRICT OF COLUMBIA	USA	Environmental Health	000	2	11/18/2021	NON-COMPETING CONTINUATION	$544,298
														Subtotal = $544,298

Issue Date FY: 2021 ( Subtotal = $506,485 )
2021	2021	GEORGETOWN UNIVERSITY (THE)	37TH AND O ST NW	WASHINGTON	DC	20057	DISTRICT OF COLUMBIA	USA	Environmental Health	000	1	2/19/2021	NEW	$506,485
														Subtotal = $506,485

Grand Total All Awards = $2,612,073

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Paternal DDT exposure and programming of metabolic dysfunction and cancer in offspring: Understanding the role of sperm mirnas and placenta development

Award Number: R01ES031611

ORGANIZATION: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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