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Characterizing three-dimensional structures at the base of primary and motile cilia using cryo-electron tomography

Award Number: F32GM137470
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

Group Awards By Issue Date FY or Funding FY:

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ABSTRACT Cilia are nearly ubiquitous among eukaryotic cells and regulate important processes including motility, sensing, and signaling. In humans, both motile and immotile (primary) cilia are critical for development and homeostasis, with disruptions in ciliary function leading to a group of diseases known as ciliopathies. While the three- dimensional structure of the axoneme of motile cilia has been well-characterized, the molecular structures of primary cilia, as well as those that comprise the base of cilia (the basal body and transition zone) remain poorly understood. Taking aim at these gaps, I will use innovative approaches, including cryo-correlative light and electron microscopy, cryo-focused ion beam milling, cryo-electron tomography, and subtomogram averaging to generate high-resolution structures of primary cilia from mouse embryonic fibroblasts and the basal body/transition zone from Chlamydomonas cells. Furthermore, I will characterize the location, structure and molecular interactions of the transition zone protein Nephrocystin-4 (NPHP4), which helps regulate molecular trafficking into and out of the cilium and causes human ciliopathies when mutated. Specifically, I will visualize and compare ciliary structures from wild type and nphp4-null Chlamydomonas and precisely analyze the size and location of nphp4-mutant structural defects. This research will further our understanding of the three- dimensional architecture and function of cilia and reveal structural changes underlying ciliary disease mechanisms.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = -$56,719 )
2023	2022	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	002	2	6/20/2023	SUPPLEMENT FOR EXPANSION	-$493
2023	2022	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	001	3	6/20/2023	NON-COMPETING CONTINUATION	-$3,944
2023	2022	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	000	3	10/27/2022	NON-COMPETING CONTINUATION	-$52,283
														Subtotal = -$56,719

Issue Date FY: 2022 ( Subtotal = $74,802 )
2022	2022	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	000	2	4/1/2022	SUPPLEMENT FOR EXPANSION	$2,500
2022	2022	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	001	3	5/31/2022	NON-COMPETING CONTINUATION	$72,302
														Subtotal = $74,802

Issue Date FY: 2021 ( Subtotal = $66,390 )
2021	2021	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	000	2	5/26/2021	NON-COMPETING CONTINUATION	$66,390
														Subtotal = $66,390

Issue Date FY: 2020 ( Subtotal = $64,926 )
2020	2020	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	001	1	5/4/2020	NEW	$0
2020	2020	UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER, THE	5323 HARRY HINES BLVD	DALLAS	TX	75390	DALLAS	USA	Biomedical Research and Research Training	000	1	5/4/2020	NEW	$64,926
														Subtotal = $64,926

Grand Total All Awards = $149,399

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Characterizing three-dimensional structures at the base of primary and motile cilia using cryo-electron tomography

Award Number: F32GM137470

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

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