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GENOMIC AND PROTEOMIC ANALYSIS OF HEROIN ADMINISTRATION

Award Number: F32DA015257
ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS
PERIOD OF PERFORMANCE START DATE: 03/01/2002
PERIOD OF PERFORMANCE END DATE: 02/28/2005

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GENOMIC AND PROTEOMIC ANALYSIS OF HEROIN ADMINISTRATION - DESCRIPTION: (Provided by Applicant): The long-term objective of this proposal is to develop a genomic and proteomic 'fingerprint' of heroin self-administration. This will be accomplished by comparing changes in mRNA and protein levels from medium spiny neurons of the nucleus accumbens (NAc) between heroin self-administering mice and yoked-heroin and saline controls. It is hypothesized that heroin administration will induce changes in mRNA and protein levels in the NAcc neurons that are specific to the means of administration, i.e. self-administration vs. yoked-controls. This hypothesis will be tested using a variety of techniques. Single cell mRNA amplification will be used to produce probes for screening Incyte cDNA GEM arrays as well as custom made 'neuroarrays'. Differential changes in mRNA seen using the arrays will be confirmed using in situ hybridization and real-time PCR. The results of these mRNA experiments will be used to identify target proteins, which will be examined using immuno-histochemistry as well as a newly developed proteomic technique, IDAT, which allows for the examination of 100s of proteins at a time. The establishment of a genomic and proteoinic fingerprint will lead to a better understanding of heroin abuse and could eventually lead to more effective treatments as well as identification of molecular risk factors associated with substance abuse.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2004 ( Subtotal = $50,548 )
2004	2004	UNIVERSITY OF OREGON	5219 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Drug Abuse National Research Service Awards for Research Training	000	3	2/19/2004	NON-COMPETING CONTINUATION	$50,548
														Subtotal = $50,548

Issue Date FY: 2003 ( Subtotal = $48,148 )
2003	2003	UNIVERSITY OF OREGON	5215 UNIVERSITY OF OREGON	EUGENE	OR	97403	LANE	USA	Drug Abuse National Research Service Awards for Research Training	000	2	7/3/2003	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$48,148
														Subtotal = $48,148

Issue Date FY: 2002 ( Subtotal = $44,212 )
2002	2002	UNIVERSITY OF PENNSYLVANIA	3451 WALNUT STREET	PHILADELPHIA	PA	19104	PHILADELPHIA	USA	Drug Abuse National Research Service Awards for Research Training	000	1	2/14/2002	NEW	$38,320
2002	2002	UNIVERSITY OF PENNSYLVANIA	3451 WALNUT STREET	PHILADELPHIA	PA	19104	PHILADELPHIA	USA	Drug Abuse National Research Service Awards for Research Training	001	1	9/28/2002	SUPPLEMENT FOR EXPANSION	$5,892
														Subtotal = $44,212

Grand Total All Awards = $142,908

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GENOMIC AND PROTEOMIC ANALYSIS OF HEROIN ADMINISTRATION

Award Number: F32DA015257

ORGANIZATION: NATIONAL INSTITUTE ON DRUG ABUSE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: FELLOWSHIP/SCHOLARSHIP/STUDENT LOANS

PERIOD OF PERFORMANCE START DATE: 03/01/2002

PERIOD OF PERFORMANCE END DATE: 02/28/2005

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