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Single-nucleus sequencing and in situ mapping of mRNA molecules in human kidney

Award Number: UH3DK114933
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Abstract The human kidney is a complex organ reported to contain over 25 cell types spatially organized into a million nephrons connected to a urine-transporting network. However, the actual cellular heterogeneity is likely to be much greater. Together, kidney structures function in heterogeneous environments created by the distinct organization (cortical- medullary) and distribution of distinct cell types. Genetic differences, injury and stress can result in kidney disease. A large gap exists in our understanding of the molecular blueprint that drives homeostatic interactions or pathology in kidney. A complete catalog of molecular signatures of all kidney cell types in 3D space, with single cell resolution, is crucial for an improved understanding of normal kidney functions, and acute and chronic kidney disease (AKD and CKD). In this project we will fill this existing gap with three specific aims. In the UG3 phase, with Aim 1, we will collect, preserve and prepare human kidney specimens from consented patients (existing and new) and optimize a single-nucleus RNA sequencing method to human kidneys. In Aim 2, we will optimize an multiplexed in situ RNA imaging method for spatially mapping the transcripts of 100+ genes within human kidney. In the UH3 phase (Aim 3), we will generate a reference cell atlas of human kidney with cell types, sub-types and their relative composition, a true 3D sub-micron resolution spatial map, and apply this knowledge to uncover molecular differences at a cellular level in CKD


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = -$171,849 )
2023	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	5	6/21/2023	NON-COMPETING CONTINUATION	-$171,849
2023	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DRIVE	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	6/20/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = -$171,849

Issue Date FY: 2021 ( Subtotal = $554,144 )
2021	2021	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	5	8/17/2021	NON-COMPETING CONTINUATION	$554,144
														Subtotal = $554,144

Issue Date FY: 2020 ( Subtotal = $803,604 )
2020	2020	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	4	8/23/2020	NON-COMPETING CONTINUATION	$700,944
2020	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	1/19/2020	EXTENSION WITH OR WITHOUT FUNDS	$102,660
														Subtotal = $803,604

Issue Date FY: 2019 ( Subtotal = $554,144 )
2019	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	8/29/2019	EXTENSION WITH OR WITHOUT FUNDS	$454,144
2019	2019	UNIVERSITY OF CALIFORNIA, SAN DIEGO	9500 GILMAN DR DEPT 621	LA JOLLA	CA	92093	SAN DIEGO	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	3	9/6/2019	SUPPLEMENT FOR EXPANSION	$100,000
														Subtotal = $554,144

Grand Total All Awards = $1,740,043

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Single-nucleus sequencing and in situ mapping of mRNA molecules in human kidney

Award Number: UH3DK114933

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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