Monday, June 30, 2025 6/30/2025

The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

Award Number: U24GM139171
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2020
PERIOD OF PERFORMANCE END DATE: 08/31/2026

Group Awards By:

View Award Description

The National Center for In-situ Tomographic Ultramicroscopy (NCITU) - Summary We propose to establish the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to be based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU will be dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of NCITU will be to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team will provide direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program will be expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. The operations at NCITU will build on already established practices at SEMC/NYSBC and benefit from the overall vibrant environment as well as the expertise of the highly trained staff already in place. NCITU will provide a full range of services for in-situ sample preparation for tomography including: culturing biological samples, plunge freezing and high pressure freezing, cryo-light microscopy (cryoLM), cryo- ultramicrotomy, focused ion beam milling (FIB-milling) including lift-out, and initial cryoTEM screening prior to submission of specimens to the network Hub for high-resolution cryoET data collection. We have already established workflows for many of these activities and have active research projects focused on expanding capabilities for automating and streamlining the entire workflow. We will continue to stay at the forefront of new developments in this area and we will incorporate new methods, technologies and workflows into the standard NCITU operating procedures as soon as these are stable and robust. While the goals of NCITU are not innately innovative, it is our intention to set the standard as one of the first world class, nationally accessible cryoET sample preparation facilities, to provide access and training that is freely and fairly available to all researchers in the US, and to continually strive to improve methods and enable science of the highest quality and value.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2024	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	002	5	6/18/2025	EXTENSION WITH OR WITHOUT FUNDS	$9,003
2025	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	4	6/18/2025	NON-COMPETING CONTINUATION	-$6,002
2025	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	001	4	6/18/2025	SUPPLEMENT FOR EXPANSION	-$3,001
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $700,000 )
2024	2024	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	002	5	9/17/2024	EXTENSION WITH OR WITHOUT FUNDS	$700,000
2024	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	4	11/17/2023	NON-COMPETING CONTINUATION	$0
2024	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	001	4	7/4/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $700,000

Issue Date FY: 2023 ( Subtotal = $1,050,000 )
2023	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	002	4	9/18/2023	NON-COMPETING CONTINUATION	$700,000
2023	2023	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	003	4	9/20/2023	SUPPLEMENT FOR EXPANSION	$350,000
2023	2022	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	3	12/30/2022	NON-COMPETING CONTINUATION	$0
2023	2022	THE NEW YORK STRUCTURAL BIOLOGY CENTER, INC.	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	001	3	2/28/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $1,050,000

Issue Date FY: 2022 ( Subtotal = $942,444 )
2022	2022	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	002	3	8/31/2022	SUPPLEMENT FOR EXPANSION	$242,444
2022	2022	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	001	3	8/30/2022	NON-COMPETING CONTINUATION	$700,000
2022	2021	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	2	5/19/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $942,444

Issue Date FY: 2021 ( Subtotal = $1,946,610 )
2021	2021	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Biomedical Research and Research Training	001	2	9/20/2021	SUPPLEMENT FOR EXPANSION	$246,610
2021	2021	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	2	8/24/2021	NON-COMPETING CONTINUATION	$1,700,000
														Subtotal = $1,946,610

Issue Date FY: 2020 ( Subtotal = $2,486,967 )
2020	2020	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Trans-NIH Research Support	000	1	9/15/2020	NEW	$1,700,000
2020	2020	NEW YORK STRUCTURAL BIOLOGY CENTER, INC., THE	89 CONVENT AVE	NEW YORK	NY	10027	NEW YORK	USA	Biomedical Research and Research Training	001	1	9/21/2020	SUPPLEMENT FOR EXPANSION	$786,967
														Subtotal = $2,486,967

Grand Total All Awards = $7,126,021

Top

All Categories

About

Search

Reports

Data Submission

Award Information

The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

Award Number: U24GM139171

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2020

PERIOD OF PERFORMANCE END DATE: 08/31/2026

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer