Saturday, September 13, 2025 9/13/2025

Dissemination of 3-photon imaging for chronic cellular imaging across species

Award Number: U01NS115585
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2020
PERIOD OF PERFORMANCE END DATE: 02/28/2026

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Dissemination of 3-photon imaging for chronic cellular imaging across species - ABSTRACT Activity-sensitive fluorescent indicators and microscopy have proven valuable tools for measuring neuronal activity, but most forms of cellular microscopy can produce images of neurons only near the brain surface and generally only after removal of tissues overlying the brain surface, such as bone. Many neurons in neocortex are out of reach of cellular microscopy. Here we propose to optimize 3-photon (3P) excitation fluorescence imaging, a form of cellular microscopy, to enable deeper imaging into the brain. Our aims are to acquire cellular information from deep cells in flies, mice, cats and macaques. After optimizing 3P microscopy, we will facilitate the duplication of our microscope and procedures in other neuroscience laboratories. We will provide open-access, detailed information on our microscope and experimental procedures in terminology that’s comprehensible to neuroscientists. In addition, we will work with one or more microscope manufacturers to ensure commercial availability of our microscope design, enabling many neuroscience laboratories to use 3P excitation to image deep into the brain with less invasive preparations, repeatedly over many days, weeks or months. To achieve our broad aims, we will pursue five specific aims:  Specific aim 1: Further optimize fast 3P excitation.  Specific aim 2: Record calcium signals from central motor circuits in intact, behaving Drosophila melanogaster.  Specific aim 3: Study sensory information processing in mouse neocortex and spatial memory in mouse hippocampus.  Specific aim 4: Study visual information processing in deep layers of cat and macaque cortex.  Specific aim 5: Share methods and facilitate availability of a commercial fast 3P microscope.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $1 )
2025	2025	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	4	8/22/2025	NON-COMPETING CONTINUATION	$1
2025	2023	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	11/21/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $1

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2023	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	6/18/2024	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $1,005,721 )
2023	2023	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	5/19/2023	NON-COMPETING CONTINUATION	$1,005,721
2023	2023	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	6/7/2023	NON-COMPETING CONTINUATION	$0
														Subtotal = $1,005,721

Issue Date FY: 2022 ( Subtotal = $1,025,583 )
2022	2022	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	2/8/2022	NON-COMPETING CONTINUATION	$923,023
2022	2022	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	2	8/17/2022	NON-COMPETING CONTINUATION	$0
2022	2022	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	3/22/2022	NON-COMPETING CONTINUATION	$102,560
														Subtotal = $1,025,583

Issue Date FY: 2021 ( Subtotal = $0 )
2021	2020	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/23/2021	NEW	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $916,785 )
2020	2020	ALLEN INSTITUTE	615 WESTLAKE AVE N	SEATTLE	WA	98109	KING	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/12/2020	NEW	$916,785
														Subtotal = $916,785

Grand Total All Awards = $2,948,090

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Dissemination of 3-photon imaging for chronic cellular imaging across species

Award Number: U01NS115585

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2020

PERIOD OF PERFORMANCE END DATE: 02/28/2026

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