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Development and dissemination of high speed 3D acousto-optic lens two-photon microscopy for in vivo imaging

Award Number: U01NS099689
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2016
PERIOD OF PERFORMANCE END DATE: 06/30/2020

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Development and dissemination of high speed 3D acousto-optic lens two-photon microscopy for in vivo imaging - To understand brain function in health and disease it is essential to rapidly monitor signaling in neural circuits. Two-photon microscopy is a core tool for neuroscience research because it enables neuronal activity to be monitored at high spatial resolution deep within brain tissue. However, the mechanical scanning and focusing of conventional designs severely limits the temporal resolution of 30 imaging and brain movement complicates recordings. We have developed a novel compact acousto-optic lens (AOL) 3D two-photon microscope that overcomes these limitations, allowing high speed imaging over volumes spanning hundreds of micrometres and real time correction of the brain movement that occurs in awake behaving animals. The agile 3D random access pointing and scanning (RAPS) at 20-40 kHz bridges the gap in the temporal resolution between optical imaging and electrophysiology. Moreover, compact design features of our AOL enable it to be added to existing two-photon microscopes at relatively low cost. This proposal aims to refine and rapidly disseminate this powerful new technology. Technology refinements include expanding the volumes that can be imaged and extending AOL-based real-time movement correction from 20 to 30. The close collaboration with experimentalists in our lab and other key leading groups distributed worldwide (to whom the technology will be initially disseminated) will ensure that current and new features of the AOL, as well as the open source GUI software, are robust and meet the requirements of the neuroscience community. This will deliver new, urgently needed technological developments required for investigating neural circuits and accelerate dissemination of this world leading technology well ahead of the 3 year timescale that is required by commercial manufacturers to bring a product to market.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2018	UNIVERSITY COLLEGE LONDON	GOWER STREET	LONDON				GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	1/28/2022	NON-COMPETING CONTINUATION	$0
2022	2018	UNIVERSITY COLLEGE LONDON	GOWER STREET	LONDON				GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	7/8/2022	SUPPLEMENT FOR EXPANSION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $534,612 )
2018	2018	UNIVERSITY COLLEGE LONDON	GOWER STREET	LONDON		WC1E 6BT		GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	7/2/2018	NON-COMPETING CONTINUATION	$437,454
2018	2018	UNIVERSITY COLLEGE LONDON	GOWER STREET	LONDON		WC1E 6BT		GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	7/3/2018	SUPPLEMENT FOR EXPANSION	$97,158
														Subtotal = $534,612

Issue Date FY: 2017 ( Subtotal = $485,483 )
2017	2017	UNIVERSITY COLLEGE LONDON	GOWER STREET	LONDON		WC1E 6BT		GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	7/3/2017	NON-COMPETING CONTINUATION	$485,483
														Subtotal = $485,483

Issue Date FY: 2016 ( Subtotal = $869,423 )
2016	2016	UNIVERSITY COLLEGE, LONDON	1-19 TORINGTON PLACE	LONDON				GBR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/22/2016	NEW	$869,423
														Subtotal = $869,423

Grand Total All Awards = $1,889,518

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Development and dissemination of high speed 3D acousto-optic lens two-photon microscopy for in vivo imaging

Award Number: U01NS099689

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2016

PERIOD OF PERFORMANCE END DATE: 06/30/2020

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