Wednesday, January 15, 2025 1/15/2025

Large-scale, simultaneous intracellular recording and stimulation of neural activity

Award Number: U01NS099687
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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We propose to develop a revolutionary tool for simultaneous intracellular recording from hundreds of single neurons in the freely-moving animal. In order to link neural activity and behavior it is essential to record the electrical activity of many individual neurons simultaneously for long periods of time. Current methods allow recording of multiple neurons only at the spike level, losing the crucial details that are available in subthreshold synaptic activity. Intracellular recordings in awake freely moving animals has been achieved only a handful of times, one neuron at a time and for very brief periods. Here we propose a novel approach for solving this problem by developing in vivo multielectrode array (MEA) platforms that implement the IN-CELL recording approach - using extracellular electrodes to provide intracellular signals – which we have developed and demonstrated using in vitro systems. The groundbreaking bioengineering process underlying the IN-CELL recording approach consists of "luring" neurons to tightly engulf micrometric sized gold mushroom-shaped microelectrode (gMµE) functionalized by bioactive materials. This unique design enables the formation of a tight seal resistance by the cleft between the neuron's plasma membrane and the gMµE “neck”, together with an increased conductance of the junctional membrane that faces the gMµE. The configuration enables effective intracellular recordings and stimulation and, in in vitro systems, is stable over long periods of time (weeks). Our unique design, experience and know-how, along with the most advanced concepts and technologies developed by the international bioengineering community for flexible in vivo MEA platforms, will be used to develop, manufacture, test and apply in vivo MEAs carrying gMµEs that match the recording and stimulation qualities of intracellular microelectrodes and will be used for acute or chronic recordings of the entire electrophysiological repertoire. The technology will be scalable and allow recordings from hundreds of individual neurons in multiple brain regions.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2020 ( Subtotal = -$1,464 )
2020	2018	HEBREW UNIVERSITY OF JERUSALEM (THE)	MOUNT SCOPUS	JERUSALEM		91905		ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	1/3/2020	NON-COMPETING CONTINUATION	-$1,464
														Subtotal = -$1,464

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2018	HEBREW UNIVERSITY OF JERUSALEM (THE)	MOUNT SCOPUS	JERUSALEM		91905		ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	2/12/2019	NON-COMPETING CONTINUATION	$0
2019	2017	HEBREW UNIVERSITY OF JERUSALEM (THE)	MOUNT SCOPUS	JERUSALEM		91905		ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	1/17/2019	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $359,203 )
2018	2018	HEBREW UNIVERSITY OF JERUSALEM (THE)	MOUNT SCOPUS	JERUSALEM		91905		ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	8/13/2018	NON-COMPETING CONTINUATION	$359,203
														Subtotal = $359,203

Issue Date FY: 2017 ( Subtotal = $359,203 )
2017	2017	HEBREW UNIVERSITY OF JERUSALEM (THE)	MOUNT SCOPUS	JERUSALEM		91905		ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	8/18/2017	NON-COMPETING CONTINUATION	$359,203
														Subtotal = $359,203

Issue Date FY: 2016 ( Subtotal = $339,605 )
2016	2016	HEBREW UNIVERSITY	**	JERUSALEM				ISR	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/20/2016	NEW	$339,605
														Subtotal = $339,605

Grand Total All Awards = $1,056,547

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Large-scale, simultaneous intracellular recording and stimulation of neural activity

Award Number: U01NS099687

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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