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Development of Protein-based Voltage Probes

Award Number: U01NS090565
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2014
PERIOD OF PERFORMANCE END DATE: 08/31/2018

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Development of Protein-based Voltage Probes - ¿ DESCRIPTION (provided by applicant): The use of genetically encoded fluorescent activity probes represent the most advanced method to monitor the electrical activity of networks of neurons without using electrodes. While genetically encoded calcium indicators have been evolved to produce robust signals in a variety of different neuronal preparations, fluorescent probes of membrane potential have not been well evolved. Current voltage probes, while finally in expanded use, will need considerable improvement if the goal of recording the activity of a large number of neurons simultaneously in vivo is to be achieved. The goal of this project is to discover protein-based fluorescent voltage probes with signal to noise characteristics that allow routine optical recording of action potentials from single cortical neurons in vivo. We are seeking probes with significantly improved signal to noise characteristics, red-shifted fluorescence spectra, faster on and off rates and better plasma membrane expression. This project brings together leading genetic probe scientists to: design new probe scaffolds, robotically screen large incrementally modified libraries of probes, identify probes with improved response properties, then validate these new probes under standardized experimental conditions in a range of 'real world' neuronal preparations with increasing levels of complexity. Finally, we will make all probe reagents (i.e. plasmid DNA, AAV particles, transgenic flies, etc.) as well as supporting validation data readily available to the research community.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2019 ( Subtotal = -$1,338 )
2019	2016	JOHN B. PIERCE LABORATORY, INC., THE	290 CONGRESS AVE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	4/10/2019	NON-COMPETING CONTINUATION	$0
2019	2016	JOHN B. PIERCE LABORATORY, INC., THE	290 CONGRESS AVE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	4/11/2019	NON-COMPETING CONTINUATION	-$1,338
														Subtotal = -$1,338

Issue Date FY: 2017 ( Subtotal = $0 )
2017	2015	JOHN B. PIERCE LABORATORY, INC., THE	290 CONGRESS AVE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	8/30/2017	SUPPLEMENT FOR EXPANSION	$0
														Subtotal = $0

Issue Date FY: 2016 ( Subtotal = $525,001 )
2016	2016	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	8/10/2016	NON-COMPETING CONTINUATION	$525,001
2016	2015	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	9/5/2016	SUPPLEMENT FOR EXPANSION	$0
														Subtotal = $525,001

Issue Date FY: 2015 ( Subtotal = $626,964 )
2015	2015	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	9/2/2015	SUPPLEMENT FOR EXPANSION	$101,963
2015	2015	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	2	9/3/2015	SUPPLEMENT FOR EXPANSION	$0
2015	2015	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	8/20/2015	NON-COMPETING CONTINUATION	$525,001
														Subtotal = $626,964

Issue Date FY: 2014 ( Subtotal = $525,001 )
2014	2014	JOHN B PIERCE FOUNDATION LABORATORY, INC	290 CONGRESS AVENUE	NEW HAVEN	CT	06519	SOUTH CENTRAL CT	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/25/2014	NEW	$525,001
														Subtotal = $525,001

Grand Total All Awards = $1,675,628

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Development of Protein-based Voltage Probes

Award Number: U01NS090565

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2014

PERIOD OF PERFORMANCE END DATE: 08/31/2018

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