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The single and same cell 3D atlas of epigenome and transcriptome of the lower urinary tract

Award Number: U01DK131377
ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/30/2021
PERIOD OF PERFORMANCE END DATE: 07/31/2026

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The single and same cell 3D atlas of epigenome and transcriptome of the lower urinary tract - In response to RFA-DK-20-013 “GenitoUrinary Development Molecular Anatomy Project (GUDMAP) - Atlas Projects”, this proposal aims “to generate data to visualize the developing urinary tract, to identify new functional and anatomical domains, and cell types”. The embryonic hindgut or cloaca progresses from a single hollow organ to two separate entities – the urinary tract and anorectal tract. The urinary tract further differentiates into the bladder rostrally and the genital urethra caudally. Guided by tissue-specific gene expression patterns, we show that several selected transcription factors and signaling molecules are critical for urinary tract development and, are responsible for some of the most complex human birth defects. However, these low-throughput and isolated case studies offer limited information; and it is difficult integrate these findings to discern gene-gene and gene-cell relationships in development and in disease. Resources including spatial annotation of all genes in all cells are needed. Using innovative technologies including single cell Multi-omics and Spatial Transcriptome, this application aims to 1) create a series of digital libraries from all cells of mouse lower urinary tract, in which every cell is annotated based on its' own transcriptomic and epigenomic profiles; 2) generate ultrahigh-density tissue- specific spatial transcriptomic map of the lower urinary tract; and 3) display spatial transcriptome at near single cell resolution onto the 3D digitalized mouse and human embryos. These resources will serve the research community to establish a comprehensive understanding of urinary tract development, including but not limited to cloaca septation and urinary tract differentiation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2024 ( Subtotal = $426,724 )
2024	2024	CEDARS-SINAI MEDICAL CENTER	8700 BEVERLY BLVD	WEST HOLLYWOOD	CA	90048	LOS ANGELES	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	4	7/16/2024	NON-COMPETING CONTINUATION	$426,724
														Subtotal = $426,724

Issue Date FY: 2023 ( Subtotal = $460,501 )
2023	2023	CEDARS-SINAI MEDICAL CENTER	8700 BEVERLY BLVD	WEST HOLLYWOOD	CA	90048	LOS ANGELES	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	3	8/3/2023	NON-COMPETING CONTINUATION	$460,501
														Subtotal = $460,501

Issue Date FY: 2022 ( Subtotal = $460,501 )
2022	2022	CEDARS-SINAI MEDICAL CENTER	8700 BEVERLY BLVD	WEST HOLLYWOOD	CA	90048	LOS ANGELES	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	001	2	7/28/2022	NON-COMPETING CONTINUATION	$460,501
2022	2021	CEDARS-SINAI MEDICAL CENTER	8700 BEVERLY BLVD	LOS ANGELES	CA	90048	LOS ANGELES	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	1/24/2022	NEW	$0
														Subtotal = $460,501

Issue Date FY: 2021 ( Subtotal = $460,501 )
2021	2021	CEDARS-SINAI MEDICAL CENTER	8700 BEVERLY BLVD	LOS ANGELES	CA	90048	LOS ANGELES	USA	Diabetes, Digestive, and Kidney Diseases Extramural Research	000	1	9/24/2021	NEW	$460,501
														Subtotal = $460,501

Grand Total All Awards = $1,808,227

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The single and same cell 3D atlas of epigenome and transcriptome of the lower urinary tract

Award Number: U01DK131377

ORGANIZATION: NATIONAL INSTITUTE OF DIABETES & DIGESTIVE & KIDNEY DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/30/2021

PERIOD OF PERFORMANCE END DATE: 07/31/2026

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