Monday, July 7, 2025 7/7/2025

T CRUZI GENOME: UU

Award Number: U01AI045061
ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES
OPDIV: NIH
AWARD CLASS: COOPERATIVE AGREEMENT
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 04/01/2000
PERIOD OF PERFORMANCE END DATE: 03/31/2005

Group Awards By:

View Award Description

T CRUZI GENOME: UU - Trypanosoma cruzi is a protozoan parasite that causes Chagas' disease, which affects several million people and constitutes a major health problem in South and Central America. No effective drug is available against this disease and no vaccine has been developed, even though basic research has been carried out into the biology of T. cruzi for decades. The large amount of useful data produced in the last years within various genome projects have demonstrated the power of this approach for gene finding and genome characterization. Therefore, in the last three years, pilot genome mapping and sequencing projects have been carried out to increase the efficiency of gene finding to identify potential drug targets and to create a knowledge base for future genome efforts. These projects have included initial karyotyping, mapping of several chromosomes, EST sequencing and sequencing of chromosome 3. The latter project as well as mapping and EST sequencing has been carried out in this laboratory. In order to take advantage of the knowledge of the genome organization of T. cruzi that has been accumulated, we here propose, together with groups at TIGR (Najib El-Sayed) and SBRI (Ken Stuart), to initiate a large effort to characterize the T. cruzi genome. The strain of choice will be the CL Brener reference strain that has been used in the initial projects. The haploid genome size of this organism is approximately 43.5 Mb. The goals of the project are: (1.) to characterize the karyotype of this strain by hybridization of 1,000 specific markers to separated chromosomes, which will result in a linkage map with a marker every 40-50 kb. (2.) to carry out end-sequencing from genomic BAC- clones in order to establish a physical map and for gene discovery. (3.) sequencing of chromosomes in T. cruzi CL Brener using clones selected on the basis of the marker and end-sequence information obtained from all three laboratories in (2.) Large-insert clone end sequencing and karyotyping will be carried out in the first year of the proposal and years 2 and 3 will be dedicated to chromosome-by-chromosome sequencing. We propose to carry out the karyotyping project as well as participate in the sequencing effort by carrying out end-sequencing from 7,000 BAC clone and complete sequencing of 10-15 Mb of genomic DNA in years 2 and 3. This project will result in a wealth of data on potential targets of drugs and vaccines and will, together with data from other parasite genome projects, be an extremely valuable resource for parasitologists world wide. We foresee that this project will bring about a plethora of investigations into gene function, gene expression, evolutionary and functional studies based on computational biology methods, and testing of potential drug targets.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2004 ( Subtotal = $535,226 )
2004	2004	KAROLINSKA INSTITUTE, RYLCAR INST OF MEDICINE & SURGERY	DEPT OF NEUROSCIENCE S-171 77	STOCKHOLM		00000		SWE	Microbiology and Infectious Diseases Research	000	5	6/21/2004	NON-COMPETING CONTINUATION	$535,226
														Subtotal = $535,226

Issue Date FY: 2003 ( Subtotal = $535,999 )
2003	2003	KAROLINSKA INSTITUTE, RYLCAR INST OF MEDICINE & SURGERY	DEPT OF NEUROSCIENCE S-171 77	STOCKHOLM		00000		SWE	Microbiology and Infectious Diseases Research	000	4	6/23/2003	NON-COMPETING CONTINUATION	$535,999
														Subtotal = $535,999

Issue Date FY: 2002 ( Subtotal = $539,700 )
2002	2002	KAROLINSKA INSTITUTE, RYLCAR INST OF MEDICINE & SURGERY	DEPT OF NEUROSCIENCE S-171 77	STOCKHOLM		00000		SWE	Microbiology and Infectious Diseases Research	000	3	9/2/2002	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$539,700
														Subtotal = $539,700

Issue Date FY: 2001 ( Subtotal = $445,371 )
2001	2001	UNIVERSITY OF UPPSALA	**	UPPSALA		00000		SWE	Microbiology and Infectious Diseases Research	000	2	6/8/2001	NON-COMPETING CONTINUATION	$445,371
														Subtotal = $445,371

Issue Date FY: 2000 ( Subtotal = $491,094 )
2000	2000	UNIVERSITY OF UPPSALA	POST OFFICE BOX 256	UPPSALA		75105		SWE	Microbiology and Infectious Diseases Research	000	1	4/1/2000	NEW	$491,094
														Subtotal = $491,094

Grand Total All Awards = $2,547,390

Top

All Categories

About

Search

Reports

Data Submission

Award Information

T CRUZI GENOME: UU

Award Number: U01AI045061

ORGANIZATION: NATIONAL INSTITUTE OF ALLERGY & INFECTIOUS DISEASES

OPDIV: NIH

AWARD CLASS: COOPERATIVE AGREEMENT

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 04/01/2000

PERIOD OF PERFORMANCE END DATE: 03/31/2005

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer