Thursday, August 28, 2025 8/28/2025

Ligand Binding and Signaling State of Anabaena Sensory Rhodopsin Transducer

Award Number: SC3GM113803
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2015
PERIOD OF PERFORMANCE END DATE: 01/31/2020

Group Awards By:

View Award Description

Ligand Binding and Signaling State of Anabaena Sensory Rhodopsin Transducer - DESCRIPTION (provided by applicant): Unlike to ion pump bacteriorhodopsin homologues, microbial sensory rhodopsin-transducer molecular complexes served a key paradigm for exploring the chemistry of protein-protein interaction in signaling cascade. In 2003, freshwater cyanobacterium Anabaena (Nostoc) sp. PCC 7120 revealed a chromosomal gene encoding a sensory rhodopsin analogue. In contrast to haloarchaeal sensory rhodopsin, it does not contain any membrane transducer gene product. However, an adjacent gene product is indicated to be a soluble transducer molecule for this photoreceptor. The detailed molecular mechanism of photoactivated receptor mediated signaling is not known. The proposed model is not related to any known microbial sensory based "phototaxis". However, it is analogous to visual photoreceptor, rhodopsin and to other G- protein coupled signaling via cytoplasmic components. Thus the role of this soluble putative transducer protein may establish a novel mode of signaling as shared by haloarchaea and eukarya. Interestingly, this putative soluble transducer molecule is present in a series of microbial species that do not contain sensory rhodopisn photoreceptor. Sequential and structural fold homology reveals such molecule without any established functional feature. Genome database search revealed their presence in broad microbial population including various pathogens. The homologue sequence of a transducer present in other sequences is referred to as "domain of unidentified function" [DUF], family. This family includes pathogens, such as Tropheryma, Actinomyces and Thermobifida. The atomic resolution structures of both photoreceptor and putative transducer are available. Structural motif based bioinformatics analysis has revealed that the transducer homologue may be classified as a member of a super family of microbial small carbohydrate binding domain. It is likely that it may serve as a novel carbohydrate binding module in a unique beta sandwich framework. Our preliminary data, based on unipolar localization suggests strong correlation and affinity to bacterial cellulose synthesis proteins that specifically localizes at pole. Recent NMR study has shown a eukaryotic like interaction of this transducer with DNA. Further, sequence- based analysis prediction along with initial data from PI's work supports the phosphor-accepting property of the transducer protein. Additionally, the phosphorylation leads to impair the unusual stability of transducer, suggesting its putative role in signaling. The unusual stable assembly and its segregation of this putative transducer protein would elucidate the signaling state of this DUF protein molecule. This project is aimed to relate the functional state of transducer as a phosphor-acceptor module and presence of a sugar binding motif and characterize the modulation of such a feature on receptor binding. The characterization of transducer's signaling state would broaden our understanding towards other DUF members including various pathogens as well.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $1,210 )
2022	2018	BETHUNE-COOKMAN UNIVERSITY, INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	000	4	9/26/2022	NON-COMPETING CONTINUATION	$1,210
														Subtotal = $1,210

Issue Date FY: 2020 ( Subtotal = -$16,314 )
2020	2018	BETHUNE-COOKMAN UNIVERSITY INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	000	4	6/19/2020	NON-COMPETING CONTINUATION	-$16,314
														Subtotal = -$16,314

Issue Date FY: 2018 ( Subtotal = $94,750 )
2018	2018	BETHUNE-COOKMAN UNIVERSITY INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	000	4	2/15/2018	NON-COMPETING CONTINUATION	$85,275
2018	2018	BETHUNE-COOKMAN UNIVERSITY INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	001	4	5/11/2018	NON-COMPETING CONTINUATION	$9,475
														Subtotal = $94,750

Issue Date FY: 2017 ( Subtotal = $94,750 )
2017	2017	BETHUNE-COOKMAN UNIVERSITY INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	001	3	6/20/2017	NON-COMPETING CONTINUATION	$9,475
2017	2017	BETHUNE-COOKMAN UNIVERSITY INC.	640 DR MARY MCLEOD BETHUNE BLVD	DAYTONA BEACH	FL	32114	VOLUSIA	USA	Biomedical Research and Research Training	000	3	1/13/2017	NON-COMPETING CONTINUATION	$85,275
														Subtotal = $94,750

Issue Date FY: 2016 ( Subtotal = $94,750 )
2016	2016	BETHUNE COOKMAN COLLEGE	640 Dr. Mary McLeod Bethune Boulev	DAYTONA BEACH	FL	32014		USA	Biomedical Research and Research Training	000	2	1/15/2016	NON-COMPETING CONTINUATION	$85,275
2016	2016	BETHUNE COOKMAN COLLEGE	640 Dr. Mary McLeod Bethune Boulev	DAYTONA BEACH	FL	32014		USA	Biomedical Research and Research Training	001	2	2/5/2016	NON-COMPETING CONTINUATION	$9,475
														Subtotal = $94,750

Issue Date FY: 2015 ( Subtotal = $94,750 )
2015	2015	BETHUNE COOKMAN COLLEGE	640 Dr. Mary McLeod Bethune Boulev	DAYTONA BEACH	FL	32014		USA	Biomedical Research and Research Training	000	1	4/28/2015	NEW	$94,750
														Subtotal = $94,750

Grand Total All Awards = $363,896

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Ligand Binding and Signaling State of Anabaena Sensory Rhodopsin Transducer

Award Number: SC3GM113803

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2015

PERIOD OF PERFORMANCE END DATE: 01/31/2020

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer