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Mapping Neural Connectivity in Zebrafish Larvae Using a Photoconvertible Protein

Award Number: SC2GM130485
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 07/01/2020
PERIOD OF PERFORMANCE END DATE: 06/30/2024

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Mapping Neural Connectivity in Zebrafish Larvae Using a Photoconvertible Protein - PROJECT SUMMARY Creation of whole-brain functional connectomes will facilitate a holistic understanding of memory formation, which is a major goal of modern neuroscience. Information gained from understanding the functional connections between neurons and the underlying plasticity between connections can be used to understand and treat neurological and mental disorders. In vivo whole-brain mapping with current technologies requires advantageous biological attributes in combination with a substantial molecular toolbox. Two invertebrate model organisms have these properties: D. melanogaster and C. elegans. However, zebrafish (Danio rerio) is the only vertebrate species with this capacity. The ability to form a functional connectome with larval zebrafish affords distinct advantages to understanding memory formation or pinpointing aberrant neural circuits in animal models of human disorders. Further, rapid development, small size, and high fecundity of zebrafish makes them an ideal organism for high- throughput screening, a useful mechanism to discover novel therapeutics for these disorders. The project will attempt to create a new tool to rapidly form in vivo whole-brain connectomes in freely moving fish with the long-term goal of understanding brain function and dysfunction at a circuit-level. Towards this goal, we will create transgenic fish that express a photoconvertible protein under the control of activity- dependent promoters. We will then determine the experimental procedures for each line of transgenic fish that most efficiently and accurately reflect the neural activity under investigation and minimize signals related to nonspecific neural activity. After we have defined the experimental potential and constraints of our transgenic lines of fish, we will seek to determine if the recorded neural activity is physiologically valid. To do so, we will form functional maps of neural activity in response to stimulation of sensory systems with previously defined neural connections. These experiments should provide the pilot data necessary to begin investigating changes in functional connectomes due to memory formation in zebrafish models of neurodevelopmental disorders. Data from the proposed studies are expected to facilitate the development of effective treatments for brain diseases and disorders, including Alzheimer's disease, PTSD, schizophrenia and autism.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2022	CSU FULLERTON AUXILIARY SERVICES CORPORATION	1121 N STATE COLLEGE BLVD	FULLERTON	CA	92831	ORANGE	USA	Biomedical Research and Research Training	000	3	1/30/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $111,090 )
2022	2022	CSU FULLERTON AUXILIARY SERVICES CORPORATION	1121 NORTH STATE COLLEGE BOULEVARD	FULLERTON	CA	92831	ORANGE	USA	Biomedical Research and Research Training	000	3	6/13/2022	NON-COMPETING CONTINUATION	$111,090
														Subtotal = $111,090

Issue Date FY: 2021 ( Subtotal = $111,091 )
2021	2021	CSU FULLERTON AUXILIARY SERVICES CORPORATION	1121 NORTH STATE COLLEGE BOULEVARD	FULLERTON	CA	92831	ORANGE	USA	Biomedical Research and Research Training	000	2	6/24/2021	NON-COMPETING CONTINUATION	$111,091
														Subtotal = $111,091

Issue Date FY: 2020 ( Subtotal = $110,985 )
2020	2020	CSU FULLERTON AUXILIARY SERVICES CORPORATION	1121 NORTH STATE COLLEGE BOULEVARD	FULLERTON	CA	92831	ORANGE	USA	Biomedical Research and Research Training	000	1	6/30/2020	NEW	$110,985
														Subtotal = $110,985

Grand Total All Awards = $333,166

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Mapping Neural Connectivity in Zebrafish Larvae Using a Photoconvertible Protein

Award Number: SC2GM130485

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 07/01/2020

PERIOD OF PERFORMANCE END DATE: 06/30/2024

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