CellScape ChipCytometry System for High-Parameter Cellular Phenotyping - Project Summary/Abstract This request is for funds to purchase a Canopy Biosciences CellScape instrument to fill a critical void in highly multiplexed spatial biology capabilities, becoming the only instrument of its kind on the Wayne State University (WSU) campus. The purchase of this instrument will directly support scientific advancement of NIH-funded research in cancer, metabolic disorders, and infectious diseases of the eye and allow our Users to perform deep analysis of various pre-clinical and patient sample types for better understanding of disease development and identifying new treatment options. The CellScape instrument will be housed within the Microscopy, Imaging, and Cytometry Resources (MICR) Core, a centralized instrumentation core facility of WSU and the Karmanos Cancer Institute (KCI). The MICR Core staff has extensive experience in all aspects of flow cytometry, confocal microscopy, and small animal imaging and currently serves over 75 PIs and 125 individual Users, both internal and external to WSU. While the MICR Core is well-equipped with several flow cytometry analyzers/sorters and light microscopy systems, the CellScape instrument will allow us to bridge these two technological arms of our core and introduce a new breadth and depth to the research capabilities of our facility. 16 PIs have been identified for the proposed instrument, 6 Major Users and 10 Minor Users. These 16 PIs have over $20M in NIH-funded direct costs from at least 5 different institutes. The new instrument will be maintained by the MICR Core which has extensive infrastructure and expertise in place to oversee User training, assay development and troubleshooting, instrument operation, data analysis, and maintenance.
