Project Summary
The objective of this RLI-S10 proposal is to acquire an electrophoresis imaging system, to enhance research and
teaching capabilities at the Western Kentucky University (WKU). An imaging system is a basic requirement in
the field of biological sciences which is commonly used for visualization, documentation and analysis of gel
electrophoresis and western blotting techniques used for DNA and protein detections. In the Department of
Biology at WKU, several investigators work on research projects that routinely need this instrument.
Additionally, several molecular biology and biochemistry laboratory courses use gel imaging system
throughout the semester for experimental analysis. These research and teaching laboratories provide hands-on
learning opportunities which can substantially enhance research quality and learning experience of a large
number of undergraduate students.
The users on this application are actively engaged in research funded by internal and external funding agencies
and have expertise with research capabilities to engage students in a wide variety of biomedical research using
different model systems and experimental analyses. The users seek funds through this proposal to replace their
18-year-old imager, Fluorchem HD2 which has limited imaging capabilities and is becoming increasingly
difficult to operate as it only runs on computers with Windows 7 that are becoming more and more scarce and
are not updatable. Moreover, the manufacturer is no longer supporting this gel imager, thus making it difficult
to change different parts of the old imager. The lack of a modern, high-resolution, functional imaging system
effects the quality of data and reliability of analyses in education and research. The acquisition of a new
ChemDoc MP imaging system from Bio-Rad with up-to-date digital imaging capability will equip researchers,
educators, and students at WKU, the ability to obtain higher resolution images of DNA and protein gels with
greater depth and accuracy.
This imaging system will be housed in the Biotechnology Center at WKU and will be available as a shared
resource that will provide several learning and training opportunities for the student community across different
departments and majors at our primarily undergraduate institute. It will enable the diverse undergraduate
population to use basic laboratory equipment and gain skills in producing high quality research data. The
equipment will fulfill the imaging needs for four different research laboratories and five different teaching
laboratories wherein undergraduate students will gain valuable firsthand learning experience through the use
of the instrument at our institute.