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SUREFECTION TECHNOLOGY FOR HIGH-THROUGHPUT PRIMARY CELL TRANSFECTION

Award Number: R44RR022955
ORGANIZATION: NATIONAL CENTER FOR RESEARCH RESOURCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2006
PERIOD OF PERFORMANCE END DATE: 11/30/2010

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SUREFECTION TECHNOLOGY FOR HIGH-THROUGHPUT PRIMARY CELL TRANSFECTION - DESCRIPTION (provided by applicant): Use of human primary cells (from health donors or patients) as physiology- or disease-relevant models provides significant advantages in basic research and therapeutic development for understanding and treating various human diseases, including cancer, cardiovascular and inflammatory diseases. Unfortunately, utility of precious primary cells is largely hindered by lack of effective means for transfection with minimal side effects, particularly in high-throughput mode. Our proposal is specifically designed to address this critical unmet need. Our Phase I studies have demonstrated that our technology is superior than existing methods in transfecting primary cells, especially fully differentiated primary cell monolayers. Phase II work is proposed to develop a 96-well high-throughput transfection system, and to further validate its applicability in extended primary cultures, including both adherent and suspension cells. Success in this project would have notable impact on basic research and therapeutic development by expanding the utility of primary cells, which are the best in vitro models to human. Success in this project will benefit public health by advancing basic research in decoding the functional roles of ~25,000 human genes, and by impacting therapeutic development for fighting diseases like cancer and inflammation.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2010 ( Subtotal = $146,875 )
2010	2010	RATIONAL BIOTECHNOLOGY INC	115 CONSTITUTION DRIVE	MENLO PARK	CA	94025	SAN MATEO	USA	Biomedical Technology	000	3	4/29/2010	SUPPLEMENT FOR EXPANSION	$146,875
2010	2009	RATIONAL BIOTECHNOLOGY INC	115 CONSTITUTION DRIVE	MENLO PARK	CA	94025	SAN MATEO	USA	Biomedical Technology	001	3	9/24/2010	NON-COMPETING CONTINUATION	$0
														Subtotal = $146,875

Issue Date FY: 2009 ( Subtotal = $654,082 )
2009	2009	RATIONAL BIOTECHNOLOGY INC	115 CONSTITUTION DRIVE	MENLO PARK	CA	94025	SAN MATEO	USA	Biomedical Technology	000	3	8/26/2009	NON-COMPETING CONTINUATION	$654,082
														Subtotal = $654,082

Issue Date FY: 2008 ( Subtotal = $1,138,726 )
2008	2008	RATIONAL BIOTECHNOLOGY INC	115 CONSTITUTION DRIVE	MENLO PARK	CA	94025	SAN MATEO	USA	Biomedical Technology	000	2	7/26/2008	COMPETING CONTINUATION	$905,300
2008	2008	RATIONAL BIOTECHNOLOGY INC	115 CONSTITUTION DRIVE	MENLO PARK	CA	94025	SAN MATEO	USA	National Center for Research Resources	001	2	9/23/2008	SUPPLEMENT FOR EXPANSION	$233,426
														Subtotal = $1,138,726

Grand Total All Awards = $1,939,683

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SUREFECTION TECHNOLOGY FOR HIGH-THROUGHPUT PRIMARY CELL TRANSFECTION

Award Number: R44RR022955

ORGANIZATION: NATIONAL CENTER FOR RESEARCH RESOURCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2006

PERIOD OF PERFORMANCE END DATE: 11/30/2010

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