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Development of technologies to advance fast and accurate tools for measuring fluorescence in living cells, with emphasis on developing assays optimized for screening in neuronal cells and brain tissue

Award Number: R44NS082222
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 01/15/2014
PERIOD OF PERFORMANCE END DATE: 08/31/2019

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Development of technologies to advance fast and accurate tools for measuring fluorescence in living cells, with emphasis on developing assays optimized for screening in neuronal cells and brain tissue - Project Summary Montana Molecular is in the business of developing and marketing genetically encoded biosensors for academic research and drug discovery in living cells. Our biosensors provide robust detection of crucial second messenger signaling, including DAG, PIP2, cAMP and Ca2+ on both fluorescence microscopes and the large installed base of automated plate readers, with metrics that match or surpass those of traditional biochemical and luminescence based assays. We have an established customer base, with over 100 early adopters in academia and industry. Our customers and industry partners have indicated a growing need for assays capable of targeting specific populations of living neurons and for “assay-ready” iPSC derived neuronal lines for use in academia and secondary drug screens. Genetically encoded assays for GPCR signaling, developed by Montana Molecular, fit seamlessly with these needs. They can be used for kinetic measurements in living cells, can be targeted to specific populations of cells in complex tissue, and have been independently validated on a wide variety of fluorescence microscopes and automated plate readers. To meet the needs of our customers and to penetrate the growing human iPSC market, we propose two specific aims. In Aim 1 we will engineer a new baculovirus vector that circumvents the payload limits and safety considerations of Lentivirus and AAV. In Aim 2, we will partner with iPSC developers in both academia and industry to create the first assay-ready iPSC derived neurons stably expressing biosensors for the detection of multiple facets of GPCR signaling.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2018	MONTANA MOLECULAR LLC	366 GALLATIN PARK DR STE A	BOZEMAN	MT	59715	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	9/26/2022	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2020 ( Subtotal = $0 )
2020	2018	MONTANA MOLECULAR LLC	366 GALLATIN PARK DR STE A	BOZEMAN	MT	59715	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	3/6/2020	NON-COMPETING CONTINUATION	$0
2020	2018	MONTANA MOLECULAR LLC	366 GALLATIN PARK DR STE A	BOZEMAN	MT	59715	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	5	3/23/2020	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2018 ( Subtotal = $210,309 )
2018	2018	MONTANA MOLECULAR LLC	366 GALLATIN PARK DR STE A	BOZEMAN	MT	59715	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	5	8/13/2018	NON-COMPETING CONTINUATION	$210,309
2018	2015	MONTANA MOLECULAR LLC	366 GALLATIN PARK DR STE A	BOZEMAN	MT	59715	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	8/13/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $210,309

Issue Date FY: 2017 ( Subtotal = $227,000 )
2017	2017	MONTANA MOLECULAR LLC	347 S FERGUSON AVE	BOZEMAN	MT	59718	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	4	9/14/2017	COMPETING CONTINUATION	$227,000
2017	2015	MONTANA MOLECULAR LLC	347 S FERGUSON AVE	BOZEMAN	MT	59718	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	1/25/2017	NON-COMPETING CONTINUATION	$0
2017	2015	MONTANA MOLECULAR LLC	347 S FERGUSON AVE	BOZEMAN	MT	59718	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	3	9/13/2017	NON-COMPETING CONTINUATION	$0
														Subtotal = $227,000

Issue Date FY: 2015 ( Subtotal = $582,394 )
2015	2015	MONTANA MOLECULAR LLC	910 TECHNOLOGY BOULEVARD	BOZEMAN	MT	59718	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	4/16/2015	NON-COMPETING CONTINUATION	$582,394
														Subtotal = $582,394

Issue Date FY: 2014 ( Subtotal = $585,457 )
2014	2014	MONTANA MOLECULAR LLC	910 TECHNOLOGY BOULEVARD	BOZEMAN	MT	59718	GALLATIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	1/13/2014	CHANGE OF GRANTEE / TRAINING INSTITUTION / AWARDING INSTITUTION	$585,457
														Subtotal = $585,457

Grand Total All Awards = $1,605,160

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Development of technologies to advance fast and accurate tools for measuring fluorescence in living cells, with emphasis on developing assays optimized for screening in neuronal cells and brain tissue

Award Number: R44NS082222

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 01/15/2014

PERIOD OF PERFORMANCE END DATE: 08/31/2019

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