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A Fully Integrated Platform for Reproducible and Scalable Single-Cell Sequencing in Clinical Research

Award Number: R44GM163418
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 06/15/2026
PERIOD OF PERFORMANCE END DATE: 05/31/2027

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A Fully Integrated Platform for Reproducible and Scalable Single-Cell Sequencing in Clinical Research - Title: A Fully Integrated Platform for Reproducible and Scalable Single-Cell Sequencing in Clinical Research Abstract: Single-cell sequencing (SC-seq) is transforming biomedical research by enabling high-resolution profiling of cellular heterogeneity, immune responses, and disease mechanisms. However, current SC-seq platforms suffer from major workflow limitations that restrict their use in clinical and translational settings. Chief among these are poor reproducibility due to variable sample preparation, limited cell retention that constrains throughput, and complex, labor-intensive workflows that are difficult to standardize across operators and sites. These challenges are particularly problematic for applications requiring detection of rare cell populations, such as immunotherapy development, precision diagnostics, and minimal residual disease detection. Sansimeon is developing a turnkey platform that integrates high-efficiency, unbiased sample preparation with scalable, deterministic cell encapsulation to support high-throughput single-cell sequencing from limited clinical specimens. Our approach unifies red blood cell and platelet removal, cell enrichment, multiplexed tagging, and CITE-seq labeling into a streamlined, one-step workflow—eliminating the need for centrifugation and minimizing hands-on time. The platform also incorporates a high-speed deterministic encapsulation system capable of processing up to 100 million cells per run with significantly improved cell-bead pairing efficiency. In Phase I, we will develop and validate a unified one-step incubation protocol for SC-seq multiplexing, CITE-seq, and cell enrichment in peripheral whole blood. In Phase II, we will build an automated benchtop instrument and an injection-molded microfluidic cartridge to enable standardized, reproducible sample preparation. We will validate the integrated system across multiple donors, operators, and timepoints, using commercial and in-house SC-seq platforms. Successful completion of this project will deliver a clinically relevant, scalable single-cell sequencing solution that improves reproducibility and expands access to single-cell technologies across immunotherapy, precision medicine, and drug discovery applications.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $382,798 )
2026	2026	SANSIMEON INC	1209 N ORANGE ST	WILMINGTON	DE	19801	NEW CASTLE	USA	Biomedical Research and Research Training	000	1	6/16/2026	NEW	$382,798
														Subtotal = $382,798

Grand Total All Awards = $382,798

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A Fully Integrated Platform for Reproducible and Scalable Single-Cell Sequencing in Clinical Research

Award Number: R44GM163418

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 06/15/2026

PERIOD OF PERFORMANCE END DATE: 05/31/2027

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