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pan-XLSM: an AI-powered microscopy platform for imaging and analysis of highly expanded tissue samples

Award Number: R44GM159519
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 08/06/2025
PERIOD OF PERFORMANCE END DATE: 06/30/2027

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pan-XLSM: an AI-powered microscopy platform for imaging and analysis of highly expanded tissue samples - Project Summary Addressing the complexities of biological function and disorders at the molecular level requires a spatial biology technology that can map the 3D distribution of molecular markers at the nanoscale within tissues. While spatial proteomics technologies have revolutionized in situ molecular profiling of tissues, limitations in resolution, epitope accessibility, sample degradation, and 3D imaging capability hinder their widespread impact. To address these issues, we developed pan-Expansion Microscopy (pan-ExM), achieving 3D multiplexed protein imaging at ~20 nm resolution in 3D ultrastructural context. Despite its promising adoption, pan-ExM is still a manual, labor-extensive, and data-intensive process, underscoring the need for automation. This is the focus of our project. Termed pan-XLSM, we propose to develop an automated fluidics light sheet microscope and a robust software platform for AI-driven image segmentation and analysis. Panluminate, Inc. is a leading company in the Spatial Biology field. Our proposed project aims to develop pan-XLSM and validate it for the characterization of mitochondria morphology disruption in kidney, liver, and brain tissues. Specifically, we propose to (1) develop a commercial prototype of an automated microfluidics axially-swept light-sheet microscope; (2) develop AI-driven image analysis software for sub-organelle segmentation; and (3) characterize mitochondria ultrastructure disruption in kidney, liver, and brain tissues as test bed applications. The developments proposed in this project will significantly enhance panluminate's existing commercial preparation service. Pan-XLSM will offer our customers a cutting-edge, automated 3D imaging and segmentation analysis module that surpasses current technologies by far. Additionally, this project will pave the way for the future commercialization of a standalone platform product.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2026 ( Subtotal = $1,083,410 )
2026	2026	PANLUMINATE INC	101 COLLEGE STREET, SUITE 210	NEW HAVEN	CT	06510	SOUTH CENTRAL CT	USA	Biomedical Research and Research Training	000	2	6/19/2026	NON-COMPETING CONTINUATION	$1,083,410
														Subtotal = $1,083,410

Issue Date FY: 2025 ( Subtotal = $1,416,590 )
2025	2025	PANLUMINATE INC	101 COLLEGE STREET, SUITE 210	NEW HAVEN	CT	06510	SOUTH CENTRAL CT	USA	Biomedical Research and Research Training	000	1	8/6/2025	NEW	$1,416,590
														Subtotal = $1,416,590

Grand Total All Awards = $2,500,000

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pan-XLSM: an AI-powered microscopy platform for imaging and analysis of highly expanded tissue samples

Award Number: R44GM159519

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 08/06/2025

PERIOD OF PERFORMANCE END DATE: 06/30/2027

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