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Automated Ultra-Long DNA and RNA Extraction for Long-read Sequencing Applications

Award Number: R44GM131530
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2018
PERIOD OF PERFORMANCE END DATE: 04/30/2024

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Automated Ultra-Long DNA and RNA Extraction for Long-read Sequencing Applications - Summary Claremont BioSolutions, LLC (CBIO) The ability to sequence and map long stretches of DNA and RNA using third generation sequencing technologies is set to transform the field of genomics and allow researchers and clinicians unparalleled insight into the complexities of our genome and diseases. However, the move to these platforms presents new hurdles in long DNA and RNA sample preparation. Most conventional spin column sample preparation methods shear DNA during purification, and although agarose plug digestion methods can isolate ultra-long DNA from samples, the protocols can be difficult, time consuming and highly variable. While newer technologies have emerged that allow for more rapid extraction of long-DNA, these kits are either limited in use to easy-to-lyse sample types (cells and blood) or require extra equipment and long processing times, which significantly increases the cost. At Claremont BioSolutions (CBio), we are developing a novel automated extraction technology that will incorporate lysis, nucleic acid binding and isolation. The technology will allow users to add and sample, hit “GO” and in 30 minutes collect DNA or RNA. In Aim 1, we will integrate our fast lysis and isolation technology into an automated cartridge to provide reproducible high quality, ultra-long DNA or RNA extraction. In Aim 2, we will validate automated ultra-long DNA extraction using easy and complex sample types, compare our technology to HMW DNA competitor's kits, and demonstrate improved long-read sequencing using two third generation technologies. In Aim 3 we will test automated RNA extraction and downstream native RNA sequencing. In Aim 4 we will perform pre-market analysis at off-site alpha and beta testing facilities to generate end-user feedback and lockdown the design. The proposed system would simplify workflow, lower cost, and reduce variability.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2021	CLAREMONT BIOSOLUTIONS LLC	1182 MONTE VISTA AVE STE 11	UPLAND	CA	91786	SAN BERNARDINO	USA	Biomedical Research and Research Training	000	2	6/17/2025	COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2022 ( Subtotal = $867,721 )
2022	2022	CLAREMONT BIOSOLUTIONS LLC	1182 MONTE VISTA AVE 11	UPLAND	CA	91786	SAN BERNARDINO	USA	Biomedical Research and Research Training	000	3	5/23/2022	NON-COMPETING CONTINUATION	$867,721
														Subtotal = $867,721

Issue Date FY: 2021 ( Subtotal = $1,164,571 )
2021	2021	CLAREMONT BIOSOLUTIONS LLC	1182 MONTE VISTA AVE 11	UPLAND	CA	91786	SAN BERNARDINO	USA	Biomedical Research and Research Training	000	2	4/7/2021	COMPETING CONTINUATION	$1,164,571
														Subtotal = $1,164,571

Grand Total All Awards = $2,032,292

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Automated Ultra-Long DNA and RNA Extraction for Long-read Sequencing Applications

Award Number: R44GM131530

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2018

PERIOD OF PERFORMANCE END DATE: 04/30/2024

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