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Electrochemically-Enhanced Plasmonic Imaging for Quantitative Proteomics

Award Number: R44GM106579
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/15/2013
PERIOD OF PERFORMANCE END DATE: 11/30/2017

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Electrochemically-Enhanced Plasmonic Imaging for Quantitative Proteomics - DESCRIPTION (provided by applicant): We are proposing a technology to help in three key areas of proteomics including (a) recognition of protein interactions, (b) characterization of post translational modifications, and (c) quantitative measurements at high spatial and/or temporal resolution to address the dynamics of protein interactions. Several significant types of protein interactions remain difficult to study with existing technologies. For example, the analysis of membrane protein interactions (mostly glycol proteins) is challenging, because these proteins are not stable outside of their native amphiphilic cellular environment. Analysis of interaction kinetics between small molecules (<500 Da, including a vast majority of metabolites and drugs) and proteins is also lacking, because these molecules are too small for fluorescence labeling, and the binding signals are too weak for label-free detection methods. Similarly problematic is the characterization of protein post-translational modifications, which alter protein behavior due to the attachment of a small functional group after translation. Specifically, we propose an electrochemically-enhanced plasmonic imaging (ECEPI) system to address key needs for quantitative analysis of protein interaction dynamics, including the ability to study membrane protein interactions in their native cellular state, characterization of small molecule interaction and post-translational modifications, measurement of interactions at high spatial and temporal resolution for the study of sub-cellular processes, and performing high-throughput analysis in multi-cellular and microarray formats. The ECEPI system relies upon careful integration of three core technologies: 1) the electrochemical surface plasmon resonance systems that have been successfully commercialized by Biosensing Instrument Inc. (BI) for their unique capabilities and solid performance, 2) a proprietary high resolution distortion-free prism-based surface plasmon resonance (SPR) imaging system currently under development at BI for high-throughput interaction analysis, and 3) a highly sensitive impedance imaging technique invented at Arizona State University. The success of this project will lead to a new instrument that is capable of: 1) Label-free real-time recognition and quantification of protein interaction kinetics; 2) Real-time characterization of post-translational modifications of proteins; 3) Quantitative measurement of small molecule interactions with proteins; 4) In situ quantification of membrane protein (and glycoprotein) interactions in their native cellular environment with cell-based assay; 5) High-resolution analysis of sub-cellular processes and; 6) High-throughput analysis in multi-cellular and microarray formats


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2018 ( Subtotal = $0 )
2018	2017	BIOSENSING INSTRUMENT INC	947 E REDFIELD RD	TEMPE	AZ	85283	MARICOPA	USA	Biomedical Research and Research Training	000	5	4/24/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $282,401 )
2017	2017	BIOSENSING INSTRUMENT INC	947 E REDFIELD RD	TEMPE	AZ	85283	MARICOPA	USA	Biomedical Research and Research Training	000	5	12/8/2016	NON-COMPETING CONTINUATION	$282,401
														Subtotal = $282,401

Issue Date FY: 2016 ( Subtotal = $560,904 )
2016	2016	BIOSENSING INSTRUMENT INC	ONE WEST ELLIOT ROAD	TEMPE	AZ	85284	MARICOPA	USA	Biomedical Research and Research Training	000	4	12/1/2015	NON-COMPETING CONTINUATION	$560,904
														Subtotal = $560,904

Issue Date FY: 2015 ( Subtotal = $559,665 )
2015	2015	BIOSENSING INSTRUMENT INC	ONE WEST ELLIOT ROAD	TEMPE	AZ	85284	MARICOPA	USA	Biomedical Research and Research Training	000	3	12/1/2014	EXTENSION WITH OR WITHOUT FUNDS	$559,665
														Subtotal = $559,665

Issue Date FY: 2014 ( Subtotal = $176,694 )
2014	2014	BIOSENSING INSTRUMENT INC	ONE WEST ELLIOT ROAD	TEMPE	AZ	85284	MARICOPA	USA	Biomedical Research and Research Training	000	2	3/26/2014	NON-COMPETING CONTINUATION	$176,694
2014	2014	BIOSENSING INSTRUMENT INC	ONE WEST ELLIOT ROAD	TEMPE	AZ	85284	MARICOPA	USA	Biomedical Research and Research Training	001	2	5/28/2014	NON-COMPETING CONTINUATION	$0
														Subtotal = $176,694

Issue Date FY: 2013 ( Subtotal = $350,000 )
2013	2013	BIOSENSING INSTRUMENT INC	ONE WEST ELLIOT ROAD	TEMPE	AZ	85284	MARICOPA	USA	Biomedical Research and Research Training	000	1	5/13/2013	NEW	$350,000
														Subtotal = $350,000

Grand Total All Awards = $1,929,664

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Electrochemically-Enhanced Plasmonic Imaging for Quantitative Proteomics

Award Number: R44GM106579

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/15/2013

PERIOD OF PERFORMANCE END DATE: 11/30/2017

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