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Automated Large and Mid Scale Nucleic Acid Preparation

Award Number: R44GM100580
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2012
PERIOD OF PERFORMANCE END DATE: 02/28/2017

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Automated Large and Mid Scale Nucleic Acid Preparation - DESCRIPTION (provided by applicant): Phase II work involves the development and optimization of a bench-top electrophoretic processing instrument and disposable cassettes that will purify milligram quantities of plasmid DNA from recombinant culture in 90 minutes. Phase I results demonstrated that 1.1 milligrams of plasmid DNA could be automatically purified from 200 mls of bacterial culture which was loaded directly into the sample well of a prototype cassette. The resulting DNA was highly pure and active as a template for automated DNA sequencing. Specific aims of Phase II include: yield optimization by refinement of the electrophoretic voltage program, optimization of lysis chemistry, refinement of the cassette design, development of the method for concentration of the purified DNA, optimization of the protocol for medium-copy-number and large-size plasmids; determination of transfection activity of purified DNA; and refinement of the method for genomic DNA purification. Phase II aims for instrument development include construction and testing of the instrument electronic circuitry and development of the operating software. The electronic components to be assembled and tested include: a microprocessor control board, circuit relay board, LCD readout, membrane touch switch, power supplies, pump and valve assembly, fluid level switch, and cooling fans; all of which will be controlled by the programmable microprocessor board and operating software. Phase II work will be carried out to finalize the cassette design and contract for construction of injection molds to create the cassettes and instrument case. A comprehensive instruction manual and specifications will be written. The bench-top instrument will have a price of ~$3000, while the disposables are projected to cost $12 per sample. MacConnell Research will be able to directly sell DNA purification cassettes and the instrument developed from this work after Phase II. The products created by this work have the potential to generate $10-20 million per year in revenue.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2017 ( Subtotal = $0 )
2017	2015	MACCONNELL RESEARCH CORPORATION	9550 WAPLES ST STE 120	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	3	6/23/2017	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2016 ( Subtotal = $0 )
2016	2015	MACCONNELL RESEARCH CORPORATION	6195 CORNERSTONE COURT #114	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	3	8/1/2016	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2015 ( Subtotal = $480,350 )
2015	2015	MACCONNELL RESEARCH CORPORATION	6195 CORNERSTONE COURT #114	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	3	1/26/2015	NON-COMPETING CONTINUATION	$480,350
														Subtotal = $480,350

Issue Date FY: 2014 ( Subtotal = $492,521 )
2014	2014	MACCONNELL RESEARCH CORPORATION	6195 CORNERSTONE COURT #114	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	2	3/24/2014	COMPETING CONTINUATION	$492,521
														Subtotal = $492,521

Grand Total All Awards = $972,871

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Automated Large and Mid Scale Nucleic Acid Preparation

Award Number: R44GM100580

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2012

PERIOD OF PERFORMANCE END DATE: 02/28/2017

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