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CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH TO IMPROVED HOT START PCR

Award Number: R44GM079836
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2007
PERIOD OF PERFORMANCE END DATE: 07/31/2010

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CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH TO IMPROVED HOT START PCR - DESCRIPTION (provided by applicant): The polymerase chain reaction (PCR) is a powerful technique used to amplify a DNA sequence of interest. Continual advancements to the technique have included development of real-time PCR technologies and reverse-transcription PCR. With the advent of these and other technologies that allow for accurate quantitation of a sequence of interest, there are continual needs for improvements to the accuracy of the technique. Herein we propose the further development of a novel Hot Start PCR strategy which may improve the specificity in PCR by reducing the number of undesired amplification products. Although numerous Hot Start PCR technologies have been developed, none of these utilize chemically-modified synthetic deoxynucleoside 5'- triphosphates (dNTPs). The present proposal aims to further develop modified dNTPs as a general solution for Hot Start activation in PCR. It is anticipated that this approach to Hot Start PCR should be amenable to existing PCR technologies, allowing for use with existing PCR systems, by simple substitution of the unmodified dNTP mix for the corresponding modified dNTP mix. In addition, we propose to further explore the utility of this technology in applications, such as single nucleotide polymorphism (SNP) detection and microarray analysis. Overall, we propose the development of a novel approach to Hot Start PCR that will offer an added level of specificity to nucleic acid amplification, with the flexibility for use in a number of PCR-based platforms.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2009 ( Subtotal = $364,714 )
2009	2009	TRILINK BIOTECHNOLOGIES INC	9955 MESA RIM ROAD	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	3	7/20/2009	NON-COMPETING CONTINUATION	$364,714
														Subtotal = $364,714

Issue Date FY: 2008 ( Subtotal = $385,248 )
2008	2008	TRILINK BIOTECHNOLOGIES INC	9955 MESA RIM ROAD	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	2	6/9/2008	COMPETING CONTINUATION	$385,248
														Subtotal = $385,248

Grand Total All Awards = $749,962

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CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH TO IMPROVED HOT START PCR

Award Number: R44GM079836

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2007

PERIOD OF PERFORMANCE END DATE: 07/31/2010

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