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DIAGNOSTIC MICROPERFUSION PLATFOM FOR FUNCTIONAL SCREENING OF THICK PREPARATIONS

Award Number: R43NS065543
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/15/2009
PERIOD OF PERFORMANCE END DATE: 07/31/2011

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DIAGNOSTIC MICROPERFUSION PLATFOM FOR FUNCTIONAL SCREENING OF THICK PREPARATIONS - DESCRIPTION (provided by applicant): The objective of the project Diagnostic Microperfusion Platform for Functional Screening of Thick Preparations is to develop a sophisticated, yet user friendly, high-throughput diagnostic instrument that provides long-term metabolic support of thick tissue samples and allows functional screening with unparallel control of culture parameters and diagnostic architecture. The first goal of the project is to extend the viability of greater than 500 ¿m thick tissue preparations and enable at least 5 days long, reproducible studies in vitro. Diffusion limited interstitial mass transport causes premature decay that is particularly exacerbated in thick samples. We propose to overcome diffusion limits by forced interstitial convection to achieve high concentration of nutrients and gas interstitially. At present, complex and expensive perfusion/recording chambers (Harvard Apparatus) allow ten-hour-long studies. Slices are thinning and their decay progress is questionable from study to study making it hard to repeat experiments, validate and interpret results. To enable successful completion of started experiments and increase the reproducibility of performed studies we will substantially mitigate culture decay, and, develop advanced fluidic architecture to aseptically isolate viable cultures and maintain unaltered perfusion if neighboring culture(s) fail. The second goal of the project is to overcome technological limitations in functional diagnostics in vitro by enabling unparallel access to thick tissue preparations. It will be up to users to select whether to monitor activity by functional imaging or electrical stimulation and recording using multi-electrode arrays. Long-term, reproducible studies will create a new benchmark for electrophysiology. Sample applications that may benefit from long-term perfusion and monitoring of neural activity are neuronal regeneration, development and plasticity as they involve processes that develop over extended periods of time. The third objective is to develop an economical fabrication approach that will turn this diagnostic platform into inexpensive, scalable, high-throughput devices with fluidic, gas, optical and electrical accessibility. Such approach will allow us to quickly and inexpensively change our designs, integrate different building blocks, and enable add-on-functionalities to satisfy specific user requirements. The end product will be a simple to use, miniature instrument whose modular architecture can be easily adjusted to suit various application challenges including but not limited to the understanding and treatment of neural and cardiac disorders, functional pharmacology in vitro, physiology, tissue engineering and transplantation. PUBLIC HEALTH RELEVANCE: Novel diagnostic platform proposed herein will advance public health by providing a simple, reliable and inexpensive test-bed for therapeutic studies. Ultimately, this development will facilitate medical and scientific discoveries that will benefit the treatment of neural and cardiac disorders.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2010 ( Subtotal = $244,894 )
2010	2010	MICROPERFUSIONS, INC.	3779 Chtthochee Summit Dr	Atlanta	GA	30339	COBB	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	2	7/21/2010	NON-COMPETING CONTINUATION	$244,894
2010	2010	MICROPERFUSIONS INC	3779 CHATTAHOOCHEE SUMMIT DR	ATLANTA	GA	30339	COBB	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	2	8/24/2010	NON-COMPETING CONTINUATION	$0
2010	2009	MICROPERFUSIONS INC	3779 CHATTAHOOCHEE SUMMIT DR	ATLANTA	GA	30339	COBB	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	1/11/2010	NEW	$0
														Subtotal = $244,894

Issue Date FY: 2009 ( Subtotal = $194,959 )
2009	2009	MICROPERFUSIONS, INC.	3779 Chtthochee Summit Dr	Atlanta	GA	30339	COBB	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	9/14/2009	NEW	$194,959
														Subtotal = $194,959

Grand Total All Awards = $439,853

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DIAGNOSTIC MICROPERFUSION PLATFOM FOR FUNCTIONAL SCREENING OF THICK PREPARATIONS

Award Number: R43NS065543

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/15/2009

PERIOD OF PERFORMANCE END DATE: 07/31/2011

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