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A Uniquely Scalable Approach to Sequence Tens of Millions of Single Cells Without Compromising Performance

Award Number: R43GM149003
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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Immunotherapies (including CAR-T cell therapies) are wonder drugs when they work, but the response rates of today’s immunotherapies are only 15-20% and therapy costs remain prohibitively high for mass adoption. Recent advances in single cell sequencing make it a critical tool for the development of next generation cell therapies through high impact applications such as TCR immune profiling and pooled CRISPR screening. Unlocking the value of these applications requires interrogation of >10 million cells per experiment. Expiration of Illumina’s core IP in 2023 re-ignited competition in US & EU markets and re-accelerated the drop in DNA sequencing costs to make sequencing of ~10 million cells affordable in 1-2 years, but existing methods for single cell barcoding do not support throughput beyond 1 million cells per experiment. Proposed alternatives are scalable, but trade-offs in performance and ease-of-use make them unsuitable for translational research. Sansimeon’s approach uniquely enables scaled single cell sequencing with throughput to 100M cells, while retaining high ease-of-use and necessary cell capture efficiency for high-throughput applications in translational research. The platform overcomes throughput limitations common to other drop-seq approaches by leveraging rapid deterministic pairing of single cells with single beads in a microchannel prior to emulsification, without the need for multi-step labeling workflows such as cell-hashing or combinatorial indexing.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $275,628 )
2023	2023	SANSIMEON INC	1209 N ORANGE ST	WILMINGTON	DE	19801	NEW CASTLE	USA	Biomedical Research and Research Training	000	1	7/26/2023	NEW	$275,628
														Subtotal = $275,628

Grand Total All Awards = $275,628

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A Uniquely Scalable Approach to Sequence Tens of Millions of Single Cells Without Compromising Performance

Award Number: R43GM149003

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

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