Radioisotope (RI) labels remain a critical tool for drug discovery applications, particularly for measuring
analytes that are not detectable by traditional optical microscopy or electrochemical methods. Drug discovery
applications require minimal perturbation of the compounds of interest, preventing the use of large labels that
significantly change the structure and properties of the compounds, particularly for small molecules. RI labels
play a fundamental role in the high-sensitivity detection of compounds as diverse as small molecule enzyme
inhibitors, receptor agonists and antagonists, carbohydrates and carbohydrate derivatives, proteins, and many
others. RIs facilitate highly sensitive detection with minimal perturbation of the size and structure of the analyte,
compared to fluorescent labels, a particularly important property in drug discovery applications. RI labels provide
unparalleled sensitivity and precision for ligand-receptor binding assays, including G-protein coupled receptor
assays. Unfortunately, the low energy and short penetration depth of most common, biologically relevant RIs
also complicate detection, limiting the capabilities for this approach.
Scintillation Nanotechnologies, INC was founded in 2018 around a series of patent-pending, radioisotope-
responsive, hybrid nanomaterials. that overcome several key limitations associated with traditional radioisotope
counting methods. Scintillation Nanotechnologies has developed a novel nanomaterial, termed nanoSPA, for
sensitive detection of low-energy radioisotopes in intracellular environments. nanoSPA presents a number of
advantages compared to other methods for imaging small molecules, including a) enhanced compatibility with
aqueous samples; b) a high-surface area to volume (SA/V) ratio to enhance ß-particle detection; c) an easily
modified surface for attachment of biomolecules and other chemical species; d) more reproducible surface
chemistry.
In this SBIR application, we propose to further develop nanoSPA with an emphasis on enhanced surface
functionalization using a diverse range of critically needed surface functionalities and to characterize the resulting
nanoSPA materials with respect to key quantitative criteria necessary to demonstrate the key commercial
potential of this platform. Once successfully developed, nanoSPA will provide an important new tool for
biomedical research.