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Sample Barcoding for Ultra High-Throughput Flow Cytometry

Award Number: R43GM140527
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 02/01/2021
PERIOD OF PERFORMANCE END DATE: 01/31/2022

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Sample Barcoding for Ultra High-Throughput Flow Cytometry - Project Summary High-throughput screens are the workhorse of the early drug discovery process. Millions of biochemical or cell-based assays are conducted to test the activity of small molecules, antibodies and oWher compoXndV Wo idenWif\ ³hiWV´ or drXg candidaWeV for fXrWher study, including pre-clinical and clinical studies. However, the most used technologies for high-throughput screening, such as HTRF, Alpha and ELISA, can only measure one or two parameters per sample, providing incompleWe informaWion of Whe drXg¶V biological mechanisms. Furthermore, these technologies detect analytes or activity outside the cell, which can result in misleading hits as compounds may fail to achieve the same effect in cell samples. To accelerate drug discovery, there is a major need for multiplexed screening at high throughput. The long-term goal of this application is to develop high-throughput flow cytometry to enable multiplexed drug screening. Although flow cytometry can measure up to 30 parameters per sample at single-cell resolution, its throughput has thus far been severely limited by its slow sampling speed. The short-term goal of Phase I is developing novel laser particle (LP) probes to barcode and pool cell samples to improve throughput, and demonstrate feasibility using a custom-built flow cytometer. This approach can eliminate sampling dead time, reducing acquisition time from 1 hour per 384-well plate to a few minutes. Furthermore, sample barcoding reduces reagent consumption and increases statistical robustness as samples are processed and stained together after pooling. Completion of Phase I research will establish the feasibility of high-throughput flow cytometry using LP barcodes. Development of this technology will greatly benefit the drug discovery industry by saving time and cost.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = $0 )
2023	2021	LASE INNOVATION INC	16 TOWER OFFICE PARK	WOBURN	MA	01801	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	5/15/2023	NEW	$0
														Subtotal = $0

Issue Date FY: 2021 ( Subtotal = $253,256 )
2021	2021	LASE INNOVATION INC.	85 BOLTON ST STE 105	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	1/27/2021	NEW	$253,256
2021	2021	LASE INNOVATION INC.	85 BOLTON ST STE 105	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Biomedical Research and Research Training	001	1	8/29/2021	NEW	$0
2021	2021	LASE INNOVATION INC.	85 BOLTON ST STE 105	CAMBRIDGE	MA	02140	MIDDLESEX	USA	Biomedical Research and Research Training	002	1	9/8/2021	NEW	$0
														Subtotal = $253,256

Grand Total All Awards = $253,256

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Sample Barcoding for Ultra High-Throughput Flow Cytometry

Award Number: R43GM140527

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 02/01/2021

PERIOD OF PERFORMANCE END DATE: 01/31/2022

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