Friday, June 20, 2025 6/20/2025

Development of Engineered Trypsin for Proteomics Sample Prep.

Award Number: R43GM134752
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2019
PERIOD OF PERFORMANCE END DATE: 08/31/2021

Group Awards By:

View Award Description

Development of Engineered Trypsin for Proteomics Sample Prep. - ABSTRACT Significance: Next generation sequencing (NGS) has has transformed the field of genomics and enabled broad adoption of the technology in biological, translational, and most recently clinical research. The proteomics equivalent to NGS has not yet been realized. As such, more accessible and cost-effective technologies for analysis of the proteome would accelerate biological and medical research. The goal of this proposal is to develop a highly-scalable digital protein analysis technology. The approach is broadly applicable and greatly improves upon cost, sensitivity, usability, and throughput of current approaches. Approach: We are developing a single molecule digital peptide sequencing platform using highly-parallelized single molecule Edman degradation and DNA encoding. Our assay generates a DNA library representing peptide sequence information on millions to billions of peptides that is sequenced on a standard NGS instrument. In this proposal, we are focused on developing reagents to expedite DNA tagging of the millions to billions of peptides for our assay by engineering proteases to cleave at modified sites. Impact: In summary, we believe our Next-Gen peptide sequencing assay will usher in the era of “next gen proteomics” effectively transforming biological, translational, and clinical research, much like NGS technology has already done in the genomics space.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2023 ( Subtotal = -$224,579 )
2023	2019	ENCODIA INC	5785 OBERLIN DR	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	3/14/2023	NEW	-$224,579
														Subtotal = -$224,579

Issue Date FY: 2022 ( Subtotal = $0 )
2022	2019	ENCODIA, INC.	11045 ROSELLE ST STE 120	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	3/7/2022	NEW	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $224,579 )
2019	2019	ENCODIA, INC	11125 FLINTKOTE AVE STE B	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	8/12/2019	NEW	$224,579
														Subtotal = $224,579

Grand Total All Awards = $0

Top

All Categories

About

Search

Reports

Data Submission

Award Information

Development of Engineered Trypsin for Proteomics Sample Prep.

Award Number: R43GM134752

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2019

PERIOD OF PERFORMANCE END DATE: 08/31/2021

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer