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Directed evolution of N-terminal amino acid binding proteins for application to protein sequencing

Award Number: R43GM123836
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2017
PERIOD OF PERFORMANCE END DATE: 08/31/2018

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Directed evolution of N-terminal amino acid binding proteins for application to protein sequencing - ABSTRACT (including UNDERLYING PREMISE and STRATEGY to ENSURE ROBUSTNESS): Our premise is that developing a better method for analyzing proteomes is required to take full advantage of the technological breakthroughs that have occurred in the last 15 years in DNA sequencing. We believe that the difficulties associated with previous proteome analysis methods can be overcome by using an entirely different approach. We propose to test this premise by obtaining affinity reagents to N-terminal amino acids that can be used in a new, massively-parallel method for protein sequencing. Our strategy to ensure a robust, unbiased and reduced risk approach is to develop in parallel the directed evolution of two protein scaffolds and compare them to commercially available and naturally-occurring IgG affinity reagents. We have chosen methods that are extremely well- established in our lab: phage display screening in both bulk and emulsions, phage display library construction, and affinity maturation. The two scaffolds for display include lipocalins and scFv Abs. While not discussed in the proposal, if there is time remaining or if a specific need arises, we may try other scaffolds.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2019 ( Subtotal = $0 )
2019	2017	ENCODIA, INC	11125 FLINTKOTE AVE STE B	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	1/25/2019	NEW	$0
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $223,600 )
2017	2017	PROCURE LIFE SCIENCES, INC.	11125 FLINTKOTE AVE STE B	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	8/9/2017	NEW	$223,600
														Subtotal = $223,600

Grand Total All Awards = $223,600

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Directed evolution of N-terminal amino acid binding proteins for application to protein sequencing

Award Number: R43GM123836

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2017

PERIOD OF PERFORMANCE END DATE: 08/31/2018

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