Thursday, July 17, 2025 7/17/2025

NOVEL METHOD FOR ASSESSING SECRETORY PROFILE OF LINEAGE SPECIFIC STEM CELLS

Award Number: R43GM080901
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2007
PERIOD OF PERFORMANCE END DATE: 10/31/2008

Group Awards By:

View Award Description

NOVEL METHOD FOR ASSESSING SECRETORY PROFILE OF LINEAGE SPECIFIC STEM CELLS - DESCRIPTION (provided by applicant): Stem cells represent unique cell populations that have the ability to undergo both self-renewal and differentiation. Although "adult" stem cells may develop into only a limited number of cell types, in contrast, early repopulating stem cells have the ability to initiate lineage-specific differentiation programs and to form numerous cell types in vitro. Although several studies have demonstrated that transcription factors play a significant role in early stem cell differentiation, extracellular hematopoietic cytokine regulatory signals appear to be more influential. Cytokine production has been extensively investigated using conventional ELISA and bioassay techniques, however, these methods typically measure cytokine secretion in cell culture supernatants, making characterization of secretory profiles of single cells difficult. Using single cell gel microdrop (GMD) encapsulation technology and flow cytometry, this SBIR aims to develop a multi- parameter single cell assay to detect cell surface marker expression and cytokine secretion simultaneously on single, viable hematopoietic stem/progenitor cells. The proposed single cell assay will provide a method for assessing the secretory profile of lineage specific stem cells and help to increase our understanding of stem cell differentiation for research and clinical applications including development of cellular therapies.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2007 ( Subtotal = $150,637 )
2007	2007	ONE CELL SYSTEMS, INC	100 INMAN STREET	CAMBRIDGE	MA	02139	MIDDLESEX	USA	Biomedical Research and Research Training	000	1	4/27/2007	NEW	$150,637
														Subtotal = $150,637

Grand Total All Awards = $150,637

Top

All Categories

About

Search

Reports

Data Submission

Award Information

NOVEL METHOD FOR ASSESSING SECRETORY PROFILE OF LINEAGE SPECIFIC STEM CELLS

Award Number: R43GM080901

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2007

PERIOD OF PERFORMANCE END DATE: 10/31/2008

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer