Wednesday, August 13, 2025 8/13/2025

CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH FOR IMPROVED HOT START PCR

Award Number: R43GM079836
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 03/01/2007
PERIOD OF PERFORMANCE END DATE: 09/30/2007

Group Awards By:

View Award Description

CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH FOR IMPROVED HOT START PCR - DESCRIPTION (provided by applicant): The polymerase chain reaction (PCR) is a powerful technique used to amplify a DNA sequence of interest. Continual advancements to the technique have included development of reverse-transcriptase PCR and real- time PCR technologies. With the advent of these and other technologies that allow for accurate quantitation of a sequence of interest, there are continual needs for improvements to the accuracy of the technique. Herein we propose the development of a novel Hot Start PCR strategy which may improve the specificity in PCR by reducing the number of undesired amplification products. Although numerous Hot Start PCR technologies have been developed, none of these utilize chemically-modified synthetic deoxynucleoside triphosphates (dNTPs). The present proposal aims to further explore the feasibility of using modified dNTPs that inhibit primer extension until a "Hot Start" preheating step is performed. It is anticipated that this approach to Hot Start PCR should be amenable to existing PCR technologies. This may allow application for use with existing PCR systems, by simple substitution of the unmodified dNTP mix with the corresponding modified dNTP mix. Overall, we propose the development of a novel approach to Hot Start PCR that will offer an added level of specificity to nucleic acid amplification. The polymerase chain reaction (PCR) is a molecular biology technique that is becoming routinely used in the field of molecular diagnostics. In this field, accurate identification of DNA sequences is imperative for reliable diagnosis of disease. To further improve the accuracy of the PCR technique, we propose the investigation of a novel Hot Start PCR approach, in which the dNTP substrates are chemically modified.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2007 ( Subtotal = $99,889 )
2007	2007	TRILINK BIOTECHNOLOGIES INC	9955 MESA RIM ROAD	SAN DIEGO	CA	92121	SAN DIEGO	USA	Biomedical Research and Research Training	000	1	2/8/2007	NEW	$99,889
														Subtotal = $99,889

Grand Total All Awards = $99,889

Top

All Categories

About

Search

Reports

Data Submission

Award Information

CHEMICALLY MODIFIED DNTPS AS A GENERAL APPROACH FOR IMPROVED HOT START PCR

Award Number: R43GM079836

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 03/01/2007

PERIOD OF PERFORMANCE END DATE: 09/30/2007

Federal Websites

Department of Health & Human Services

HHS Operating Divisions

HHS Staff Divisions

Download A Document Viewer