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3D Printed Multifunctional Brain Windows for Simultaneous Optical Imaging and Electrophysiology

Award Number: R42NS110165
ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 09/01/2019
PERIOD OF PERFORMANCE END DATE: 05/31/2026

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3D Printed Multifunctional Brain Windows for Simultaneous Optical Imaging and Electrophysiology - PROJECT SUMMARY This proposal responds to PAR-18-870 titled “BRAIN Initiative: Development Optimization, and Validation of Novel Tools and Technologies for Neuroscience Research (STTR).” This project is a Fast Track STTR with a phase I and phase II. The small business, Applied Universal Dynamics Corp. (AUD) is collaborating with the University of Minnesota (U of MN). The activity patterns of tens of millions of neurons – all organized into circuits distributed across multiple brain regions mediate our interaction with the outside world. A mechanistic understanding of the neuronal underpinnings of sensory perception, action, emotion, and cognition requires measuring activities of these neuronal circuits at single cell resolution across several millimeters, and at multiple temporal scales temporal scales. We propose to engineer and commercially disseminate transparent polymer skulls that simultaneously allow wide-field optical neural sensing along with high temporal resolution electrical recordings from the whole mammalian cortex. We will build on key technologies developed in in our laboratories. Specifically, our collaborative has developed a methodology to design and fabricate transparent skulls (Brain Windows) that allow sub-cellular resolution imaging of structure and function of the whole dorsal cortex in behaving mice. In PHASE I of this proposal, we will first utilize 3-dimensional printing methodologies to functionalize these transparent skulls with 8 channels of transparent electrocorticogram (ECoG) recording electrodes (Aim 1). These devices will be utilized to perform simultaneous mesoscale calcium imaging and ECoG recordings (Aim 2). In PHASE II of this proposal, we will refine the 3D printing methodology to realize ultra-high density, transparent 128 channel μECoG arrays (Aim 1) for simultaneous whole cortex ECoG and cellular resolution calcium imaging (Aim 2). Finally, we will engineer versions of the technology that allow simultaneous extracellular recordings with surface ECoG and whole cortex optical imaging to realize a platform that allows true 3D brain activity mapping (Aim 3).


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2025 ( Subtotal = $0 )
2025	2023	APPLIED UNIVERSAL DYNAMICS, CORP	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	5/9/2025	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2024 ( Subtotal = $0 )
2024	2019	APPLIED UNIVERSAL DYNAMICS, CORP	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	10/23/2023	NEW	$0
														Subtotal = $0

Issue Date FY: 2023 ( Subtotal = $738,079 )
2023	2023	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	3	5/31/2023	NON-COMPETING CONTINUATION	$738,079
														Subtotal = $738,079

Issue Date FY: 2022 ( Subtotal = $755,288 )
2022	2022	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	004	2	9/12/2022	EXTENSION WITH OR WITHOUT FUNDS	$0
2022	2022	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	2	5/31/2022	EXTENSION WITH OR WITHOUT FUNDS	$755,288
2022	2022	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	003	2	6/8/2022	EXTENSION WITH OR WITHOUT FUNDS	$0
2022	2021	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	1	6/1/2022	SUPPLEMENT FOR EXPANSION	$0
2022	2019	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	002	1	6/2/2022	NEW	$0
														Subtotal = $755,288

Issue Date FY: 2021 ( Subtotal = $95,058 )
2021	2021	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	001	1	6/28/2021	SUPPLEMENT FOR EXPANSION	$95,058
2021	2019	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	6/28/2021	NEW	$0
														Subtotal = $95,058

Issue Date FY: 2020 ( Subtotal = $0 )
2020	2019	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	3/10/2020	NEW	$0
														Subtotal = $0

Issue Date FY: 2019 ( Subtotal = $224,759 )
2019	2019	APPLIED UNIVERSAL DYNAMICS, CORP.	23875 CHESTNUT DR	LORETTO	MN	55357	HENNEPIN	USA	Extramural Research Programs in the Neurosciences and Neurological Disorders	000	1	8/23/2019	NEW	$224,759
														Subtotal = $224,759

Grand Total All Awards = $1,813,184

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3D Printed Multifunctional Brain Windows for Simultaneous Optical Imaging and Electrophysiology

Award Number: R42NS110165

ORGANIZATION: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 09/01/2019

PERIOD OF PERFORMANCE END DATE: 05/31/2026

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