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Single Cell Analysis via Nanoscale Tip-Enhanced Laser Ablation Mass Spectrometry

Award Number: R42GM106454
ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
OPDIV: NIH
AWARD CLASS: DISCRETIONARY
AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)
PERIOD OF PERFORMANCE START DATE: 05/01/2013
PERIOD OF PERFORMANCE END DATE: 04/30/2018

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Single Cell Analysis via Nanoscale Tip-Enhanced Laser Ablation Mass Spectrometry - ¿ DESCRIPTION (provided by applicant): The goal of the proposed Phase II STTR research is to develop an imaging system capable of detecting and identifying biomolecules in cells and tissue sections with sub-cellular spatial resolution. The system combines an atomic force microscope (AFM) with mass spectrometry (MS) analysis and utilizes a unique tip-enhanced laser ablation (TELA) method developed in the Phase I portion of the research. The current technology limitation of imaging mass spectrometry is the fact that it is possible to obtain high-resolution images of small molecules and low-resolution images of large molecules, but it is not possible to both at once. The proposed instrument directly addresses this technology limitation. The TELA effect developed under Phase I as an off - line MS method will be developed under Phase II into an on-line instrument that integrates AFM and mass spectrometry imaging for both excellent spatial resolution and biomolecule mass spectrometry. The aims of the project are divided into 1) further refinement of TELA and development of the on-line TELA-MS interface, 2) integration of TELA and MS imaging, and 3) application to brain imaging. Refinement of the tip - enhanced laser ablation involves a study of the fundamental interaction between the pulsed ablation laser and the atomic force microscope tip. The optimized ablation system will be combined with an ultra-low flow electrospray ionization system in which charged particles from the electrospray source interact with the ablation plume to form biomolecule ions. Integration of the AFM and MS imaging systems will use four imaging modalities: spot-by-spot sampling, area sampling, area imaging, and depth profiling. The result is an AFM capable of both physical and chemical imaging of cells and tissue. The AFM TELA-MS device will be applied to detection of biomolecules in rat brain tissue. Spatial profiling of lipids, peptides, cocaine, and cocaine metabolites will be performed. The AFM TELA-MS system will be constructed by Anasys, based on past product development and manufacturing experience with nanoscale imaging systems such as atomic force microscopy and infrared spectroscopy chemical imaging using tunable pulsed laser sources. Mass spectrometry performance tests will be carried out on in the laboratory of Prof. Kermit Murray at Louisiana State University who is an expert in the development of laser ablation methods for the detection of biomolecules by mass spectrometry.


Issue Date FY	Funding FY	Legal Entity Name	Legal Entity Address	Legal Entity City	Legal Entity State	Legal Entity Zip Code	Legal Entity COUNTY	Legal Entity COUNTRY	Assistance Listing	Award Code	Budget Year	Action Date	Action Type	Action Amount

Issue Date FY: 2018 ( Subtotal = $0 )
2018	2017	ANASYS INSTRUMENTS CORP.	325 CHAPALA ST.	SANTA BARBARA	CA	93101	SANTA BARBARA	USA	Biomedical Research and Research Training	000	3	9/16/2018	NON-COMPETING CONTINUATION	$0
														Subtotal = $0

Issue Date FY: 2017 ( Subtotal = $586,526 )
2017	2017	ANASYS INSTRUMENTS CORP.	325 CHAPALA ST.	SANTA BARBARA	CA	93101	SANTA BARBARA	USA	Biomedical Research and Research Training	000	3	4/26/2017	NON-COMPETING CONTINUATION	$586,526
														Subtotal = $586,526

Issue Date FY: 2016 ( Subtotal = $597,820 )
2016	2016	ANASYS INSTRUMENTS CORPORATION	121 GRAY AVENUE	SANTA BARBARA	CA	93101	SANTA BARBARA	USA	Biomedical Research and Research Training	000	2	4/27/2016	COMPETING CONTINUATION	$597,820
														Subtotal = $597,820

Grand Total All Awards = $1,184,346

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Single Cell Analysis via Nanoscale Tip-Enhanced Laser Ablation Mass Spectrometry

Award Number: R42GM106454

ORGANIZATION: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

OPDIV: NIH

AWARD CLASS: DISCRETIONARY

AWARD ACTIVITY TYPE: SCIENTIFIC/HEALTH RESEARCH (INCLUDES SURVEYS)

PERIOD OF PERFORMANCE START DATE: 05/01/2013

PERIOD OF PERFORMANCE END DATE: 04/30/2018

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